A Comparative Analyzing of Zooplankton Community Diversity in Surface Layer Water of Reservoir Via eDNA Metabarcoding and Microscopy

We compared two sampling methods, eDNA metabarcoding and microscope identification (MSI), for the analysis of zooplankton diversity in reservoirs with its inflow and outflow streams. The dynamic patterns of Cladocera and Rotifera at different time points were similar between the two sampling methods...

Full description

Saved in:
Bibliographic Details
Published inDiversity (Basel) Vol. 14; no. 10; p. 797
Main Authors Ji, Chang Woo, Oh, Hye-Ji, Chang, Kwang-Hyeon, Park, Young-Seuk, Kwak, Ihn-Sil
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.09.2022
Subjects
Aquatic ecosystems
Cladocera
Classification
community
Comparative analysis
Copepoda
diversity
DNA barcoding
eDNA
Environmental aspects
Genera
Genetic aspects
Identification
Laboratories
Methods
microscope identification
Morphology
Plankton
Population density
Reservoirs
RNA
Rotifera
rRNA 18S
Sampling
Sampling methods
Streams
Surface layers
Taxonomy
Water inflow
Water outflow
Water temperature
Zooplankton
South Korea
United States > US
Online AccessGet full text

Cover

More Information
Summary:We compared two sampling methods, eDNA metabarcoding and microscope identification (MSI), for the analysis of zooplankton diversity in reservoirs with its inflow and outflow streams. The dynamic patterns of Cladocera and Rotifera at different time points were similar between the two sampling methods, but there was a slight difference in the Copepoda. Specifically, the members of the Copepoda subclass could not be easily classified using the MSI method, whereas eDNA metabarcoding could detect minor taxa of Cladocera and Rotifera. Upon comparing the list of zooplankton communities in Korea with the gene database of NCBI, only ~56% of the zooplankton genera reported in Korea could be detected based on the 18S rRNA gene. However, eDNA metabarcoding detected a more diverse range of zooplankton despite the lack of genetic information. As water temperature increased after May, the zooplankton diversity decreased according to the MSI method but increased according to the eDNA metabarcoding method. Although eDNA metabarcoding has some limitations, it was able to detect a wider diversity of zooplankton compared to the MSI. eDNA metabarcoding provides a more reliable means to identify zooplankton.
ISSN:1424-2818
1424-2818
DOI:10.3390/d14100797