Fluorogenic Hand-Held Immunoassay for the Identification of Ricin: Rapid Analyte Measurement Platform

Rapid analyte measurement platform (RAMP™) fluorogenic hand-held immunoassays (HHAs) were evaluated for inclusivity/sensitivity, exclusivity/specificity, sample matrix effects, ruggedness/stability, and reproducibility in detection of ricin (RCA 60 ), a potential biological threat agent. The limit o...

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Bibliographic Details
Published inJournal of immunoassay & immunochemistry Vol. 28; no. 3; pp. 227 - 241
Main Authors Fulton, R. E., Thompson, H. G.
Format Journal Article
LanguageEnglish
Published England Taylor & Francis Group 01.01.2007
Subjects
Biological threat agent
Fluorescence
Hand-held assay
Immunoassay - instrumentation
Immunoassay - methods
Immunochromatographic assay
RAMP
Rapid analyte measurement platform
Reproducibility of Results
Ricin
Ricin - analysis
Ricinus communis
Sensitivity and Specificity
Time Factors
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Summary:Rapid analyte measurement platform (RAMP™) fluorogenic hand-held immunoassays (HHAs) were evaluated for inclusivity/sensitivity, exclusivity/specificity, sample matrix effects, ruggedness/stability, and reproducibility in detection of ricin (RCA 60 ), a potential biological threat agent. The limit of detection of HHAs for RCA 60 was 14 ng/mL or approximately 140 pg/test. HHAs were inclusive in detection of ricin RCA 60 , RCA 120 , ricin A chain, and ricin B chain and exclusive in discrimination of RCA 60 from other toxins. None of the sample matrices tested affected assay performance. RAMP™ ratios were tolerant of increases in sample volume, however, a decrease in sample volume of 25% resulted in significantly increased readings. RAMP™ readings were highly reproducible lot-to-lot, cartridge-to-cartridge, day-to-day, and reader-to-reader.
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ISSN:1532-1819
1532-4230
DOI:10.1080/15321810701454730