use of Verticillium dahliae and Diplodia scrobiculata to induce resistance in Pinus halepensis against Diplodia pinea infection

• Published in: European journal of plant pathology Vol. 120; no. 4; pp. 331 - 337
• Main Authors: Muñoz, Zaida, Moret, Assumpció, Garcés, Sandra
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.04.2008; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Agriculture; Biological and medical sciences; Biological control; Biomedical and Life Sciences; Dieback; Diplodia; Diplodia pinea; disease resistance; Ecology; Full Research Paper; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Fungi; induced resistance; Life Sciences; Phytopathology. Animal pests. Plant and forest protection; Pinus halepensis; Plant diseases; Plant Pathology; Plant resistance; Plant Sciences; shoots; Spain; stems; Tip blight; trees; Verticillium; Verticillium albo-atrum; Verticillium dahliae; Europe; Spain; Aleppo pine; Fungi; Tip blight; Induced resistance; Plant pathology; Pinus halepensis; Verticillium dahliae; Microbiology; Plant pathogen; Fungi·Induced resistance; Biological control; Diplodia; Pinus pinea; Pathogenicity; Plant; Treatment; Softwood forest tree; Gymnospermae; Coniferales; Spermatophyta; Fungi Imperfecti
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-007-9221-x

The pathogenic fungi Verticillium alboatrum and Diplodia scrobiculata were assayed for biological control of Diplodia pinea on Aleppo pine (Pinus halepensis) in Catalonia (north-eastern Spain). Young shoots were pre-treated with inoculations of either V. dahliae or D. scrobiculata, by placing colonized agar plugs on wounds made by removing a single needle fascicle. An inoculation with D. pinea was performed 15 days later. Two months after the shoot inoculations, the canker length on the stems was measured and the percentage of shoot dieback calculated. Verticillium dahliae and D. scrobiculata were found to significantly reduce the canker length of D. pinea (P < 0.05) when compared with positive controls. Diplodia pinea was slightly more sensitive to V. dahliae than to D. scrobiculata, but no significant differences (P > 0.05) were observed in the mean canker length between the two treatments. Trees pre-inoculated with V. dahliae resulted in 31.12% shoot dieback, while those pre-inoculated with D. scrobiculata resulted in 32.18% shoot dieback, compared with positive controls (42.85%).