Binding of Actinomycin D to mRNA In Vivo and In Vitro
When rats received an intraperitoneal injection of [3H]actinomycin D, it bound to the RNA moiety of free and bound polysomes of rat liver. The labeling increased gradually up to 6 h or 6+6h.3 The poly(A)-containing mRNA showed definite radioactivity and its specific activity was higher than that of...
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Published in | Journal of biochemistry (Tokyo) Vol. 93; no. 2; pp. 361 - 366 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
01.02.1983
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Subjects | |
Online Access | Get full text |
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Summary: | When rats received an intraperitoneal injection of [3H]actinomycin D, it bound to the RNA moiety of free and bound polysomes of rat liver. The labeling increased gradually up to 6 h or 6+6h.3 The poly(A)-containing mRNA showed definite radioactivity and its specific activity was higher than that of rRNA, although the total radioactivity of rRNA was markedly higher than that of poly(A)-containing mRNAs. An equilibrium dialysis method using rabbit globin mRNA showed that the binding constant of actinomycin D to globin mRNA was 0.056×106 M−1, and globin mRNA had 2 binding sites per mol for actinomycin D. From the results of the present experiments and those described in the preceding paper, it is suggested that one of the mechanisms by which actinomycin D treatment in vivo and in vitro stimulates the template activity of mRNA may be binding to mRNA, which alters the conformation of mRNA. |
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Bibliography: | istex:5BC2685E0BEB04BCF9A04B375C1CAF2DB7314BD2 1Present address: Department of Biochemistry, School of Medicine, University of the Ryukyus, Naha, Okinawa 902. ark:/67375/HXZ-2HS7KTPB-2 2To whom correspondence should be addressed ArticleID:93.2.361 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-924X 1756-2651 |
DOI: | 10.1093/oxfordjournals.jbchem.a134188 |