Pyrophosphate and fructose 2,6-bisphosphate effects on glycolysis in pea seed extracts

• Published in: Plant physiology (Bethesda) Vol. 76; no. 2; pp. 316 - 320
• Main Authors: Smyth, Douglas A., Wu, Min-Xian, Black, Clanton C.
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.10.1984
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Crassulacean acid metabolism; Dehydrogenases; Diphosphates; Economic plant physiology; Enzyme activity; Enzymes; fosfatos; fructosa; fructose; Fundamental and applied biological sciences. Psychology; glicolisis; glycolyse; Glycolysis; graine; Leaves; Metabolism; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Peas; phosphate; Phosphates; Photosynthesis, respiration. Anabolism, catabolism; pisum sativum; Plant physiology and development; seeds; semilla; Glycolysis; Spermatophyta; Leguminosae; Dicotyledones; Pisum sativum; Angiospermae
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.76.2.316

The participation of pyrophosphate-dependent phosphofructokinase (PPi-PFK) in plant glycolysis was examined using extracts from pea seeds (Pisum sativum L. cv Alaska). Glycolysis starting with fructose 6-phosphate was measured under aerobic conditions as the accumulation of pyruvate. Pyruvate accumulated in a medium containing PPi and adenosine diphosphate at about two-thirds of the rate in a medium containing adenosine diphosphate and adenosine triphosphate (ATP). The PPi-dependent pyruvate accumulation had the same reactant requirements and sensitivity to glycolysis inhibitors, sodium fluoride, and iodoacetamide, as the well-established ATP-dependent glycolysis. Added fructose 2,6-bisphosphate stimulated both the PPi-dependent pyruvate accumulation and PPi-PFK activity whereas this modulator had no effect on ATP-dependent glycolysis or ATP-PFK. Collectively these results demonstrate a PPi-dependent glycolytic pathway in plants which is responsive to fructose 2,6-bisphosphate.