Crevicular fluid levels of plasma glutathione peroxidase (eGPx) in periodontal health and disease

• Published in: Archives of oral biology Vol. 54; no. 6; pp. 543 - 548
• Main Authors: Patel, Swati Pradeep, Pradeep, A.R, Chowdhry, Shikha
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2009
• Subjects: Adult; Advanced Basic Science; Alveolar Bone Loss - classification; Alveolar Bone Loss - enzymology; Biomarkers - analysis; Case-Control Studies; Chronic Periodontitis - blood; Chronic Periodontitis - enzymology; Chronic Periodontitis - therapy; Dental Scaling; Dentistry; Female; Gingival crevicular fluid; Gingival Crevicular Fluid - enzymology; Gingivitis - blood; Gingivitis - enzymology; Glutathione peroxidase; Glutathione Peroxidase - blood; Humans; Male; Oxidative stress; Oxidative Stress - physiology; Periodontal Attachment Loss - classification; Periodontal Attachment Loss - enzymology; Periodontal Diseases - blood; Periodontal Diseases - enzymology; Periodontal Diseases - therapy; Periodontal Index; Periodontal Pocket - classification; Periodontal Pocket - enzymology; Periodontitis; Periodontium - enzymology; Root Planing; Glutathione peroxidase; Oxidative stress; Periodontitis; Gingival crevicular fluid
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2009.02.002

Abstract Background & objectives Reactive oxygen species (ROS) have been implicated in numerous human diseases, including periodontal diseases. Plasma glutathione peroxidase (eGPx) as an important antioxidant, has a protective role against ROS and is an established marker of oxidative stress. The present study evaluated the levels of eGPx in GCF to further probe into the role of oxidants and antioxidants in periodontal disease. Methods 60 subjects were divided into three groups consisting of 20 subjects in each group based on gingival index, pocket probing depth, clinical attachment loss and radiological parameters (bone loss): healthy (group 1), gingivitis (group 2) and periodontitis (group 3), whilst, group 3 patients after the treatment constituted group 4. GCF samples were collected from all groups to estimate the levels of eGPx using ELISA. Results The mean eGPx concentration in GCF were observed to be the highest in group 3 i.e., 30.89 ± 4.93 ng/μl and lowest in group 1 i.e., 15.32 ± 3.06 ng/μl. The mean eGPx concentration in group 2 (23.77 ± 2.91 ng/μl) and group 4 (18.92 ± 3.53 ng/μl) fell between the highest and the lowest values. Conclusion This suggests that eGPx levels in GCF increase proportionally with the severity of periodontal diseases. eGPx can be considered as a marker of oxidative stress in periodontal diseases. However, controlled, longitudinal studies with larger samples have to be carried out to confirm this possibility.