High pressure disruption: a two-step treatment for selective extraction of intracellular components from the microalga Porphyridium cruentum

The microalga Porphyridium cruentum is known to produce many components of interest. One of them is B-Phycoerythrin (B-PE), a water-soluble intracellular pigment used as an immunofluorescent probe. Current methods to extract this molecule involve total cell disruption and lead to a mix of all the wa...

Full description

Saved in:
Bibliographic Details
Published inJournal of applied phycology Vol. 25; no. 4; pp. 983 - 989
Main Authors Jubeau, Sebastien, Marchal, Luc, Pruvost, Jeremy, Jaouen, Pascal, Legrand, Jack, Fleurence, Joel
Format Journal Article
LanguageEnglish
Published Dordrecht Springer-Verlag 01.08.2013
Springer Netherlands
Springer Nature B.V
Springer Verlag
Subjects
Biomedical and Life Sciences
Chemical and Process Engineering
culture media
Distilled water
Ecology
Engineering Sciences
Freshwater & Marine Ecology
High pressure
Life Sciences
Microalgae
Plant Physiology
Plant Sciences
Porphyridium cruentum
proteins
Rhodophyta
High-pressure disruption
Selective extraction
B-Phycoerythrin
Online AccessGet full text

Cover

More Information
Summary:The microalga Porphyridium cruentum is known to produce many components of interest. One of them is B-Phycoerythrin (B-PE), a water-soluble intracellular pigment used as an immunofluorescent probe. Current methods to extract this molecule involve total cell disruption and lead to a mix of all the water-soluble components. Subsequently, the pigment purification is very complex. An alternative approach to extract B-PE selectively and thus simplify the purification procedure has been developed using a high-pressure cell disrupter. Different pressures (from 27 to 270 MPa), extracting mediums (distilled water and original microalgae culture medium), and numbers of passages (1 to 3) have been tested. Proteins are selectively more extracted than B-PE at low pressure in original medium. It is thus possible to remove part of the intracellular proteins in a first step and then recover enriched B-Phycoerythrin fraction at higher pressure in distilled water.
Bibliography:http://dx.doi.org/10.1007/s10811-012-9910-5
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-012-9910-5