Assessment of DFG-S19 method for the determination of common endocrine disruptor pesticides in wine samples with an estimation of the uncertainty of the analytical results

• Published in: Food chemistry Vol. 138; no. 1; pp. 54 - 61
• Main Authors: Pelit, Füsun Okçu, Ertaş, Hasan, Seyrani, Işıl, Nil Ertaş, F.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.05.2013; Elsevier
• Subjects: Biological and medical sciences; Chromatography, Gas - methods; Endocrine disruptor; Endocrine Disruptors - analysis; Fermented food industries; Food Contamination - analysis; Food industries; Fundamental and applied biological sciences. Psychology; Gas chromatography; Limit of Detection; Pesticide Residues - analysis; Pesticides; Sample preparation; Vitaceae; Wine; Wine - analysis; Wines and vinegars; Wine; Gas chromatography; Endocrine disruptor; Sample preparation; Pesticides; Alcoholic beverage; Uncertainty; Sample; Estimation; Method
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.10.053

► A number of different methods was modified on the basis of original DFG-S19 method. ► Each methodology was compared with respect to the validation parameters. ► The method including the replacement GPC with florosil column was chosen. ► This method is efficient, reliable, robust, cheap and being adopted for wine analysis. ► The uncertainty for the determination of the pesticides was also calculated. A gas chromatographic method for the determination of endocrine disruptor pesticides (Chlorpyrifos, Penconazole, Procymidone, Iprodione, Bromopropylate and Lambda-Cyhalothrin) in wine samples is described. A general DFG-S19 method for residual pesticide determination in all kind of food stuff was investigated to simplify and adopt for wine samples in this work. Alternative sample preparation routes were elucidated and compared according to their recovery values. Four different separation techniques were tested and the method employing florosil column after a LLE procedure was applied for wine samples with satisfactory recovery ratios (72–97%). The pesticides were extracted from the sample by cyclohexane–ethyl acetate mixture (1:1 v/v) and cleaned up by florosil column. The regression coefficients were at least 0.99 and relative standard deviations were no higher than 16%. Detection limits were in the range of 0.6–2.9ng/mL and the relative expanded measurement uncertainties were calculated in the 7–22% range.