A novel fluorescent labeling compound for GluN2A containing N-methyl-d-aspartate receptors identified by autodisplay-based screening

[Display omitted] •Ligand binding domains of the NMDA receptor were surface displayed on E. coli.•Displaying cells are applicable for binding assays to identify novel binders.•Fluorescent compound 8 binds to the same binding site as GluN2A selective TCN-201.•Compound 8 is applicable for staining of...

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Published inJournal of pharmaceutical analysis Vol. 14; no. 7; pp. 100945 - 4
Main Authors Dombovski, Alexander, Steigerwald, Ruben, Ritter, Nadine, Disse, Paul, Goerges, Gunnar, Osthues, Jana, Aymanns, Isabel, Dilkaute, Carina, Schreiber, Julian, Düfer, Martina, Seebohm, Guiscard, Wünsch, Bernhard, Jose, Joachim
Format Journal Article
LanguageEnglish
Published China Elsevier B.V 01.07.2024
Xi'an Jiaotong University, Journal of Pharmaceutical Analysis
Xi'an Jiaotong University
Elsevier
Subjects
Antibodies
Binding sites
Chemistry
Experiments
Flow cytometry
Fluorescence microscopy
Glutamate receptors
Glutamic acid receptors (ionotropic)
Labeling
Ligands
N-Methyl-D-aspartic acid receptors
Pharmaceutical sciences
Short Communication
Transmembrane domains
Visualization
Germany
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Summary:[Display omitted] •Ligand binding domains of the NMDA receptor were surface displayed on E. coli.•Displaying cells are applicable for binding assays to identify novel binders.•Fluorescent compound 8 binds to the same binding site as GluN2A selective TCN-201.•Compound 8 is applicable for staining of recombinant and native NMDA receptors.•Compound 8 exhibits selectivity for GluN2A over GluN2B containing receptors.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2095-1779
2214-0883
2214-0883
DOI:10.1016/j.jpha.2024.01.013