New insights into comprehensive analysis of magnesium transporter (MGT) gene family in rice (Oryza sativa L.)
Magnesium transporters (MGTs) regulate magnesium absorption, transport, and redistribution in higher plants. To investigate the role of the Oryza sativa MGTs gene family members under salt stress, this study analyzed the protein properties, gene structure, phylogenetic relationship, synteny patterns...
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Published in | 3 Biotech Vol. 13; no. 10; p. 322 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.10.2023
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Magnesium transporters (MGTs) regulate magnesium absorption, transport, and redistribution in higher plants. To investigate the role of the
Oryza sativa MGTs
gene family members under salt stress, this study analyzed the protein properties, gene structure, phylogenetic relationship, synteny patterns, expression, and co-expression networks of 23 non-redundant
OsMGT
. The evolutionary relationship of the OsMGT gene family was fully consistent with their functional domain, and were divided into three main classes based on the conserved domain: MMgT, CorA-like, and NIPA. The α/β patterns in the protein structures were highly similar in the CorA-like and NIPA members, with the conserved structures in the Mg
2+
-binding and catalytic regions. The CorA-like clade-related proteins demonstrated the highest numbers of protein channels with Pro, Ser, Lys, Gly, and Tyr, as the critical binding residues. The expression analysis of
OsMGT
genes in various tissues showed that
MGTs
’ gene family may possess critical functions during rice development. Gene expression analysis of candidate
OsMGT
using reverse-transcription quantitative real-time PCR (RT-qPCR) found that four
OsMGT
genes exhibited different expression patterns in salt-sensitive and salt-tolerant rice genotypes. We hypothesize that the
OsMGT
gene family members may be involved in responses to salt stress. These findings could be useful for further functional investigation of
MGTs
as well as defining their involvement in abiotic stress studies. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2190-572X 2190-5738 |
DOI: | 10.1007/s13205-023-03735-4 |