In vitro and in vivo evaluation of antioxidant activity of Petasites japonicus Maxim. flower buds extracts

The antioxidant activity of Petasites japonicus flower buds cultivated in Tokushima, Japan, was examined in vitro and in vivo. The flower bud extracts were assayed using either oxygen radical absorbance capacity or 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Antioxidants in the...

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Published inBioscience, biotechnology, and biochemistry Vol. 84; no. 3; pp. 621 - 632
Main Authors Hiemori-Kondo, Miki, Nii, Mika
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 03.03.2020
Oxford University Press
Subjects
Animals
Antioxidant activity
Antioxidants - pharmacology
caffeoylquinic acid
Chromatography, High Pressure Liquid - methods
Flowers - chemistry
fukinolic acid
In Vitro Techniques
Male
Mice, Inbred C57BL
Mice, Inbred ICR
Oxidative Stress - drug effects
Petasites - chemistry
Petasites japonicus flower bud
Plant Extracts - pharmacology
Tandem Mass Spectrometry
Thiobarbituric Acid Reactive Substances - metabolism
caffeoylquinic acid
fukinolic acid
flower bud
Antioxidant activity
Petasites japonicus flower bud
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Summary:The antioxidant activity of Petasites japonicus flower buds cultivated in Tokushima, Japan, was examined in vitro and in vivo. The flower bud extracts were assayed using either oxygen radical absorbance capacity or 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Antioxidants in the 80% ethanol extract were investigated using online high-performance liquid chromatography-DPPH and were identified as caffeic acid, 3-O-caffeoylquinic acid, fukinolic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid using liquid chromatography-mass spectrometry. Fukinolic acid was the most active compound based on its activity and abundance. Administering the extracts orally to ICR mice prior to iron injection significantly suppressed plasma thiobarbituric acid reactive substance (TBARS) production. Moreover, TBARS and triglyceride concentrations in the plasma of C57BL/6 mice fed with a high fat diet were also significantly decreased by the extract. The results suggest that antioxidative compounds in P. japonicus can be used in the management of oxidative stress. In vitro and in vivo evaluation of the antioxidant activity of Petasites japonicus Maxim. flower buds extracts
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ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2019.1691913