Doehlert design-desirability function multi-criteria optimal separation of 17 phenolic compounds from extra-virgin olive oil by capillary zone electrophoresis

• Published in: Food chemistry Vol. 146; pp. 558 - 568
• Main Authors: Ballus, Cristiano Augusto, Meinhart, Adriana Dillenburg, de Souza Campos, Francisco Alberto, Bruns, Roy Edward, Godoy, Helena Teixeira
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.03.2014; Elsevier
• Subjects: Biological and medical sciences; Brazil; Capillary zone electrophoresis; Derringer’s desirability function; Doehlert matrix; Electrophoresis, Capillary - instrumentation; Electrophoresis, Capillary - methods; Extra-virgin olive oil; Food toxicology; Hydrogen-Ion Concentration; Medical sciences; Method optimization; Olive Oil; Phenolic compounds; Phenols - chemistry; Phenols - isolation & purification; Plant Oils - chemistry; Toxicology; Brazil; Doehlert matrix; Method optimization; Capillary zone electrophoresis; Phenolic compounds; Derringer’s desirability function; Extra-virgin olive oil; Zone electrophoresis; Separation; Derringer's desirability function; Vegetable oil; Olive oil; Edible oil; Optimization; Virgin oil; Design; Phenols
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.09.102

•A CE-DAD method to separate 17 phenolic compounds from olive oil was optimised.•Resolution, runtime and migration time RSD values were evaluated as responses.•This was the first optimisation study to evaluate 37 responses simultaneously.•Derringer’s desirability function was used to simultaneously optimise the responses.•The method was validated and applied to 15 samples found in Brazilian supermarkets. In Brazil, the consumption of extra-virgin olive oil (EVOO) is increasing annually, but there are no experimental studies concerning the phenolic compound contents of commercial EVOO. The aim of this work was to optimise the separation of 17 phenolic compounds already detected in EVOO. A Doehlert matrix experimental design was used, evaluating the effects of pH and electrolyte concentration. Resolution, runtime and migration time relative standard deviation values were evaluated. Derringer’s desirability function was used to simultaneously optimise all 37 responses. The 17 peaks were separated in 19min using a fused-silica capillary (50μm internal diameter, 72cm of effective length) with an extended light path and 101.3mmolL−1 of boric acid electrolyte (pH 9.15, 30kV). The method was validated and applied to 15 EVOO samples found in Brazilian supermarkets.