Induction of myoblast proliferation in L6 myoblast cultures by fetal serum of double-muscled and normal cattle

Double-muscled (DM) cattle possess more muscle fibers than do normal-muscled (NM) beef or dairy cattle. Serum-borne growth factors have been shown to modulate myogenesis. Media containing serum from 12 DM and 60 NM fetuses grouped by crown-rump lengths (CRL) of 25, 26 to 50, 51 to 75, or 75 cm were...

Full description

Saved in:
Bibliographic Details
Published inJournal of animal science Vol. 71; no. 6; p. 1464
Main Authors Gerrard, D.E, Judge, M.D
Format Journal Article
LanguageEnglish
Published United States 01.06.1993
Subjects
Animals
BOVIN
Breeding
Cattle - embryology
Cattle - genetics
Cell Division
Clone Cells
CRECIMIENTO
CROISSANCE
CULTIVO DE TEJIDOS
CULTURE DE TISSU
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
DOSAGE BIOLOGIQUE
Embryonic and Fetal Development
ENSAYO BIOLOGICO
Female
Fetal Blood - physiology
FETO
FIBRAS MUSCULARES
FIBRE MUSCULAIRE
FOETUS
GANADO BOVINO
HORMONAS
HORMONE
LECTINA
LECTINE
Male
Muscles - cytology
Muscles - embryology
Rats
SERUM SANGUIN
SISTEMA OSEOMUSCULAR
SQUELETTE ET MUSCULATURE
SUERO SANGUINEO
Online AccessGet more information

Cover

More Information
Summary:Double-muscled (DM) cattle possess more muscle fibers than do normal-muscled (NM) beef or dairy cattle. Serum-borne growth factors have been shown to modulate myogenesis. Media containing serum from 12 DM and 60 NM fetuses grouped by crown-rump lengths (CRL) of 25, 26 to 50, 51 to 75, or 75 cm were used to test the effect of fetal serum on L6 myoblast proliferation. Because DM and NM fetuses were similar in CRL at ages corresponding to 20- to 60-cm CRL, CRL was used to determine fetal age. Normal-muscled fetal serum-induced thymidine incorporation in L6 myoblasts was greater (P 0.05) at CRL 50- than at 50-cm CRL. Mean incorporation tended to increase with CRL. Thymidine incorporation was 56, 41, and 41% greater (P 0.05) with serum from DM fetuses than with that from NM fetuses at CRL of 25, 26 to 50, and 51 to 75 cm, respectively. Morphological examination of cross-sections of the semitendinosus muscle showed that apparent muscle fiber number was greater (P 0.05) for DM fetuses than for NM fetuses. These results confirm greater apparent muscle fiber number in DM cattle and show the existence of greater growth factor activity in serum of DM fetuses during early fetal development. This greater growth factor activity may play a role in bovine muscle fiber hyperplasia
Bibliography:L70
L50
9425088
ISSN:0021-8812
1525-3163
DOI:10.2527/1993.7161464x