Lignocellulosic biomass as a carbohydrate source for lipid production by Mortierella isabellina

• Published in: Bioresource technology Vol. 128; pp. 385 - 391
• Main Authors: Zeng, Jijiao, Zheng, Yubin, Yu, Xiaochen, Yu, Liang, Gao, Difeng, Chen, Shulin
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.01.2013; Elsevier
• Subjects: Aldehydes; Biological and medical sciences; Carbohydrate Metabolism - physiology; Derivatives; Food industries; Fundamental and applied biological sciences. Psychology; Fungi; Hydrolysates; Inhibitors; Lignin - metabolism; Lignocellulose; Lipids; Lipids - biosynthesis; Microorganisms; Mortierella - metabolism; Mortierella isabellina; Single-cell oil; Sugars; Use and upgrading of agricultural and food by-products. Biotechnology; Wheat straw hydrolysate; Wheat straw hydrolysate; Inhibitors; Mortierella isabellina; Single-cell oil; Zygomycota; Cereal by product; Lipids; Lignocellulosics; Biomass; Mortierella; Fungi; Oil; Hydrolysate; Wheat straw; Carbohydrate; Inhibitor; Microorganism
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.10.079

► Characterized capability of Mortierella isabellina for lipid production on various type of carbohydrates. ► Investigated inhibitory effects of lignocelluosic derivatives on cell biomass production and lipid accumulation. ► Investigated the growth kinetics of M. isabellina by using wheat-straw hydrolysate after dilute acid pretreatment. Various carbon sources including monosugars, disaccharides and carboxymethyl-cellulose (CMC) were used for single-cell oil production by the filamentous fungus Mortierella isabellina. In addition, the inhibitory effects of lignocellulose-derived compounds (lignin aldehydes, furan aldehydes and weak acid) were investigated. C6 sugars were preferably used for growth compared to C5 sugars. CMC was not an usable substrate, implying the absence of a cellulase system in this fungus. Lignin derivatives showed the most inhibitory effects, but acetic and formic acids at concentrations of 4g/L improved lipid production, achieving 6.81±0.07g/L and 6.66±0.33g/L respectively, which was twice as high as that of the control. A 16.8% lipid yield from hydrolysate suggested that this fungus could be useful for microbial lipid production.