Sirtuin 6 protects human retinal pigment epithelium cells from LPS-induced inflammation and apoptosis partly by regulating autophagy
Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on LPS-induced retinal injury. ARPE-19 cells were incubated with LPS to induce inflammation. The cell viability was determined using CCK-8 assay....
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Published in | Bioscience, biotechnology, and biochemistry Vol. 84; no. 10; pp. 2062 - 2068 |
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02.10.2020
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Abstract | Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on LPS-induced retinal injury. ARPE-19 cells were incubated with LPS to induce inflammation. The cell viability was determined using CCK-8 assay. The mRNA level and protein expression of corresponding genes was detected using qRT-PCR and western blot, respectively. The production of inflammatory cytokines was measured using ELISA kit. The levels of oxidative stress-related factors were measured using their detection kits. Cell apoptosis was observed using TUNEL assay. The results showed that Sirt6 was downregulated after LPS treatment. Sirt6 strengthened LPS-induced autophagy by promoting the expression of LC3II/I, beclin1 and ATG5. Sirt6 treatment significantly inhibited LPS-induced inflammation, oxidative stress and cell apoptosis, which was then partly abolished by 3 MA. These results suggest Sirt6 to be an important regulator for LPS-induced inflammation, oxidative stress, and apoptosis partly by regulating cell autophagy.
A schematic model of the role and potential mechanism of Sirt6 in LPS-induced retinal 20 diseases. |
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AbstractList | Abstract
Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on LPS-induced retinal injury. ARPE-19 cells were incubated with LPS to induce inflammation. The cell viability was determined using CCK-8 assay. The mRNA level and protein expression of corresponding genes was detected using qRT-PCR and western blot, respectively. The production of inflammatory cytokines was measured using ELISA kit. The levels of oxidative stress-related factors were measured using their detection kits. Cell apoptosis was observed using TUNEL assay. The results showed that Sirt6 was downregulated after LPS treatment. Sirt6 strengthened LPS-induced autophagy by promoting the expression of LC3II/I, beclin1 and ATG5. Sirt6 treatment significantly inhibited LPS-induced inflammation, oxidative stress and cell apoptosis, which was then partly abolished by 3 MA. These results suggest Sirt6 to be an important regulator for LPS-induced inflammation, oxidative stress, and apoptosis partly by regulating cell autophagy.
GRAPHICAL ABSTRACT
A schematic model of the role and potential mechanism of Sirt6 in LPS-induced retinal 20 diseases. Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on LPS-induced retinal injury. ARPE-19 cells were incubated with LPS to induce inflammation. The cell viability was determined using CCK-8 assay. The mRNA level and protein expression of corresponding genes was detected using qRT-PCR and western blot, respectively. The production of inflammatory cytokines was measured using ELISA kit. The levels of oxidative stress-related factors were measured using their detection kits. Cell apoptosis was observed using TUNEL assay. The results showed that Sirt6 was downregulated after LPS treatment. Sirt6 strengthened LPS-induced autophagy by promoting the expression of LC3II/I, beclin1 and ATG5. Sirt6 treatment significantly inhibited LPS-induced inflammation, oxidative stress and cell apoptosis, which was then partly abolished by 3 MA. These results suggest Sirt6 to be an important regulator for LPS-induced inflammation, oxidative stress, and apoptosis partly by regulating cell autophagy. Abstract Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on LPS-induced retinal injury. ARPE-19 cells were incubated with LPS to induce inflammation. The cell viability was determined using CCK-8 assay. The mRNA level and protein expression of corresponding genes was detected using qRT-PCR and western blot, respectively. The production of inflammatory cytokines was measured using ELISA kit. The levels of oxidative stress-related factors were measured using their detection kits. Cell apoptosis was observed using TUNEL assay. The results showed that Sirt6 was downregulated after LPS treatment. Sirt6 strengthened LPS-induced autophagy by promoting the expression of LC3II/I, beclin1 and ATG5. Sirt6 treatment significantly inhibited LPS-induced inflammation, oxidative stress and cell apoptosis, which was then partly abolished by 3 MA. These results suggest Sirt6 to be an important regulator for LPS-induced inflammation, oxidative stress, and apoptosis partly by regulating cell autophagy. Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on LPS-induced retinal injury. ARPE-19 cells were incubated with LPS to induce inflammation. The cell viability was determined using CCK-8 assay. The mRNA level and protein expression of corresponding genes was detected using qRT-PCR and western blot, respectively. The production of inflammatory cytokines was measured using ELISA kit. The levels of oxidative stress-related factors were measured using their detection kits. Cell apoptosis was observed using TUNEL assay. The results showed that Sirt6 was downregulated after LPS treatment. Sirt6 strengthened LPS-induced autophagy by promoting the expression of LC3II/I, beclin1 and ATG5. Sirt6 treatment significantly inhibited LPS-induced inflammation, oxidative stress and cell apoptosis, which was then partly abolished by 3 MA. These results suggest Sirt6 to be an important regulator for LPS-induced inflammation, oxidative stress, and apoptosis partly by regulating cell autophagy. A schematic model of the role and potential mechanism of Sirt6 in LPS-induced retinal 20 diseases. |
Author | Liu, Jingjing Liu, Dan |
Author_xml | – sequence: 1 givenname: Jingjing surname: Liu fullname: Liu, Jingjing email: liujjing2020@163.com organization: The First Affiliated Hospital of Jinzhou Medical University – sequence: 2 givenname: Dan surname: Liu fullname: Liu, Dan organization: The First Affiliated Hospital of Jinzhou Medical University |
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Cites_doi | 10.1038/nature04724 10.4161/auto.36184 10.1089/ars.2013.5371 10.1016/j.it.2015.04.003 10.1111/bph.14428 10.3389/fncel.2019.00154 10.1038/srep20639 10.1016/j.bbrc.2018.01.159 10.1038/eye.2015.100 10.1038/s41419-018-1065-0 10.1152/physrev.00021.2004 10.1155/2010/190724 10.1371/journal.pone.0004160 10.1074/jbc.R112.402768 10.1111/iej.12742 10.3390/ijms19082279 10.1080/15548627.2017.1384886 10.1186/s13148-016-0224-3 10.4161/auto.4600 10.1016/j.cell.2010.01.028 10.1155/2012/597514 10.1007/s12031-017-1005-y 10.1016/j.neuroscience.2017.09.035 10.1016/j.jaut.2013.06.011 10.4049/jimmunol.1701573 10.1007/s10565-016-9371-8 10.1016/j.molimm.2008.12.001 10.1007/s12272-017-0989-8 10.1146/annurev.nutr.012809.104635 10.1089/ars.2014.5994 |
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Keywords | apoptosis autophagy inflammation Sirtuin 6 human retinal pigment epithelium cells |
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References | Mohamad Nasir (2021033109285064400_cit0010) 2018; 64 Carafa (2021033109285064400_cit0009) 2016; 8 Iachettini (2021033109285064400_cit0027) 2018; 9 Lee (2021033109285064400_cit0011) 2018; 51 Leung (2021033109285064400_cit0006) 2009; 46 Kaarniranta (2021033109285064400_cit0017) 2017; 33 Simo (2021033109285064400_cit0001) 2010; 2010 Perez (2021033109285064400_cit0003) 2015; 36 Hara (2021033109285064400_cit0026) 2006; 441 Zhang (2021033109285064400_cit0020) 2017; 366 Sebastian (2021033109285064400_cit0013) 2012; 287 Liu (2021033109285064400_cit0030) 2016; 6 Bermudez (2021033109285064400_cit0025) 2019; 13 Wu (2021033109285064400_cit0016) 2018; 17 Zeng (2021033109285064400_cit0015) 2018; 19 Bae (2021033109285064400_cit0012) 2017; 40 Mitter (2021033109285064400_cit0031) 2014; 10 Bazan (2021033109285064400_cit0005) 2011; 31 Lappas (2021033109285064400_cit0021) 2012; 2012 Strauss (2021033109285064400_cit0002) 2005; 85 Parzych (2021033109285064400_cit0014) 2014; 20 Mizushima (2021033109285064400_cit0024) 2010; 140 Hu (2021033109285064400_cit0022) 2018; 175 Perez (2021033109285064400_cit0004) 2013; 45 Wang (2021033109285064400_cit0028) 2018; 14 Wang (2021033109285064400_cit0029) 2009; 4 Parmar (2021033109285064400_cit0018) 2018; 200 Ratnayaka (2021033109285064400_cit0007) 2015; 29 Mizushima (2021033109285064400_cit0023) 2007; 3 Abais (2021033109285064400_cit0019) 2015; 22 Feng (2021033109285064400_cit0008) 2018; 496 |
References_xml | – volume: 441 start-page: 885 issue: 7095 year: 2006 ident: 2021033109285064400_cit0026 article-title: Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice publication-title: Nature doi: 10.1038/nature04724 contributor: fullname: Hara – volume: 10 start-page: 1989 issue: 11 year: 2014 ident: 2021033109285064400_cit0031 article-title: Dysregulated autophagy in the RPE is associated with increased susceptibility to oxidative stress and AMD publication-title: Autophagy doi: 10.4161/auto.36184 contributor: fullname: Mitter – volume: 20 start-page: 460 issue: 3 year: 2014 ident: 2021033109285064400_cit0014 article-title: An overview of autophagy: morphology, mechanism, and regulation publication-title: Antioxid Redox Signal doi: 10.1089/ars.2013.5371 contributor: fullname: Parzych – volume: 36 start-page: 354 issue: 6 year: 2015 ident: 2021033109285064400_cit0003 article-title: Immune mechanisms in inflammatory and degenerative eye disease publication-title: Trends Immunol doi: 10.1016/j.it.2015.04.003 contributor: fullname: Perez – volume: 175 start-page: 3594 issue: 17 year: 2018 ident: 2021033109285064400_cit0022 article-title: MicroRNA-351-5p aggravates intestinal ischaemia/reperfusion injury through the targeting of MAPK13 and Sirtuin-6 publication-title: Br J Pharmacol doi: 10.1111/bph.14428 contributor: fullname: Hu – volume: 13 start-page: 154 year: 2019 ident: 2021033109285064400_cit0025 article-title: Lipopolysaccharide-induced autophagy mediates retinal pigment epithelium cells survival. modulation by the phospholipase D pathway publication-title: Front Cell Neurosci doi: 10.3389/fncel.2019.00154 contributor: fullname: Bermudez – volume: 6 start-page: 20639 year: 2016 ident: 2021033109285064400_cit0030 article-title: Impairing autophagy in retinal pigment epithelium leads to inflammasome activation and enhanced macrophage-mediated angiogenesis publication-title: Sci Rep doi: 10.1038/srep20639 contributor: fullname: Liu – volume: 17 start-page: 3607 issue: 3 year: 2018 ident: 2021033109285064400_cit0016 article-title: Autophagy regulates TGF-beta2-induced epithelial-mesenchymal transition in human retinal pigment epithelium cells publication-title: Mol Med Rep contributor: fullname: Wu – volume: 496 start-page: 1148 issue: 4 year: 2018 ident: 2021033109285064400_cit0008 article-title: Autophagy activated by SIRT6 regulates Abeta induced inflammatory response in RPEs publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2018.01.159 contributor: fullname: Feng – volume: 29 start-page: 1013 issue: 8 year: 2015 ident: 2021033109285064400_cit0007 article-title: Dementia of the eye: the role of amyloid beta in retinal degeneration publication-title: Eye (Lond) doi: 10.1038/eye.2015.100 contributor: fullname: Ratnayaka – volume: 9 start-page: 996 issue: 10 year: 2018 ident: 2021033109285064400_cit0027 article-title: Pharmacological activation of SIRT6 triggers lethal autophagy in human cancer cells publication-title: Cell Death Dis doi: 10.1038/s41419-018-1065-0 contributor: fullname: Iachettini – volume: 85 start-page: 845 issue: 3 year: 2005 ident: 2021033109285064400_cit0002 article-title: The retinal pigment epithelium in visual function publication-title: Physiol Rev doi: 10.1152/physrev.00021.2004 contributor: fullname: Strauss – volume: 2010 start-page: 190724 year: 2010 ident: 2021033109285064400_cit0001 article-title: The retinal pigment epithelium: something more than a constituent of the blood-retinal barrier–implications for the pathogenesis of diabetic retinopathy publication-title: J Biomed Biotechnol doi: 10.1155/2010/190724 contributor: fullname: Simo – volume: 4 start-page: e4160 issue: 1 year: 2009 ident: 2021033109285064400_cit0029 article-title: Autophagy and exosomes in the aged retinal pigment epithelium: possible relevance to drusen formation and age-related macular degeneration publication-title: PLoS One doi: 10.1371/journal.pone.0004160 contributor: fullname: Wang – volume: 287 start-page: 42444 issue: 51 year: 2012 ident: 2021033109285064400_cit0013 article-title: From sirtuin biology to human diseases: an update publication-title: J Biol Chem doi: 10.1074/jbc.R112.402768 contributor: fullname: Sebastian – volume: 51 start-page: e74 issue: Suppl 2 year: 2018 ident: 2021033109285064400_cit0011 article-title: Sirtuin 6 attenuates periapical lesion propagation by modulating hypoxia-induced chemokine (C-C motif) ligand 2 production in osteoblasts publication-title: Int Endod J doi: 10.1111/iej.12742 contributor: fullname: Lee – volume: 19 start-page: 8 year: 2018 ident: 2021033109285064400_cit0015 article-title: Hedgehog signaling pathway and autophagy in cancer publication-title: Int J Mol Sci doi: 10.3390/ijms19082279 contributor: fullname: Zeng – volume: 14 start-page: 518 issue: 3 year: 2018 ident: 2021033109285064400_cit0028 article-title: Aberrant SIRT6 expression contributes to melanoma growth: role of the autophagy paradox and IGF-AKT signaling publication-title: Autophagy doi: 10.1080/15548627.2017.1384886 contributor: fullname: Wang – volume: 8 start-page: 61 year: 2016 ident: 2021033109285064400_cit0009 article-title: Sirtuin functions and modulation: from chemistry to the clinic publication-title: Clin Epigenetics doi: 10.1186/s13148-016-0224-3 contributor: fullname: Carafa – volume: 3 start-page: 542 issue: 6 year: 2007 ident: 2021033109285064400_cit0023 article-title: How to interpret LC3 immunoblotting publication-title: Autophagy doi: 10.4161/auto.4600 contributor: fullname: Mizushima – volume: 140 start-page: 313 issue: 3 year: 2010 ident: 2021033109285064400_cit0024 article-title: Methods in mammalian autophagy research publication-title: Cell doi: 10.1016/j.cell.2010.01.028 contributor: fullname: Mizushima – volume: 2012 start-page: 597514 year: 2012 ident: 2021033109285064400_cit0021 article-title: Anti-inflammatory properties of sirtuin 6 in human umbilical vein endothelial cells publication-title: Mediators Inflamm doi: 10.1155/2012/597514 contributor: fullname: Lappas – volume: 64 start-page: 157 issue: 2 year: 2018 ident: 2021033109285064400_cit0010 article-title: Emerging roles of Sirtuin 6 in Alzheimer’s disease publication-title: J Mol Neurosci doi: 10.1007/s12031-017-1005-y contributor: fullname: Mohamad Nasir – volume: 366 start-page: 95 year: 2017 ident: 2021033109285064400_cit0020 article-title: Sirtuin 6 protects the brain from cerebral ischemia/reperfusion injury through NRF2 activation publication-title: Neuroscience doi: 10.1016/j.neuroscience.2017.09.035 contributor: fullname: Zhang – volume: 45 start-page: 7 year: 2013 ident: 2021033109285064400_cit0004 article-title: The eye: A window to the soul of the immune system publication-title: J Autoimmun doi: 10.1016/j.jaut.2013.06.011 contributor: fullname: Perez – volume: 200 start-page: 3128 issue: 9 year: 2018 ident: 2021033109285064400_cit0018 article-title: Lipocalin 2 plays an important role in regulating inflammation in retinal degeneration publication-title: J Immunol doi: 10.4049/jimmunol.1701573 contributor: fullname: Parmar – volume: 33 start-page: 113 issue: 2 year: 2017 ident: 2021033109285064400_cit0017 article-title: Autophagy regulates death of retinal pigment epithelium cells in age-related macular degeneration publication-title: Cell Biol Toxicol doi: 10.1007/s10565-016-9371-8 contributor: fullname: Kaarniranta – volume: 46 start-page: 1374 issue: 7 year: 2009 ident: 2021033109285064400_cit0006 article-title: Bacterial endotoxin activates retinal pigment epithelial cells and induces their degeneration through IL-6 and IL-8 autocrine signaling publication-title: Mol Immunol doi: 10.1016/j.molimm.2008.12.001 contributor: fullname: Leung – volume: 40 start-page: 1380 issue: 12 year: 2017 ident: 2021033109285064400_cit0012 article-title: Sirtuin 6, a possible therapeutic target for type 2 diabetes publication-title: Arch Pharm Res doi: 10.1007/s12272-017-0989-8 contributor: fullname: Bae – volume: 31 start-page: 321 year: 2011 ident: 2021033109285064400_cit0005 article-title: Docosahexaenoic acid signalolipidomics in nutrition: significance in aging, neuroinflammation, macular degeneration, Alzheimer’s, and other neurodegenerative diseases publication-title: Annu Rev Nutr doi: 10.1146/annurev.nutr.012809.104635 contributor: fullname: Bazan – volume: 22 start-page: 1111 issue: 13 year: 2015 ident: 2021033109285064400_cit0019 article-title: Redox regulation of NLRP3 inflammasomes: ROS as trigger or effector? publication-title: Antioxid Redox Signal doi: 10.1089/ars.2014.5994 contributor: fullname: Abais |
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Snippet | Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on... Abstract Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of... |
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SubjectTerms | apoptosis Apoptosis - drug effects autophagy Autophagy - drug effects Cell Line human retinal pigment epithelium cells Humans inflammation Inflammation - chemically induced Inflammation - metabolism Lipopolysaccharides - pharmacology Oxidative Stress - drug effects Retinal Pigment Epithelium - cytology Sirtuin 6 Sirtuins - metabolism |
Title | Sirtuin 6 protects human retinal pigment epithelium cells from LPS-induced inflammation and apoptosis partly by regulating autophagy |
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