Sirtuin 6 protects human retinal pigment epithelium cells from LPS-induced inflammation and apoptosis partly by regulating autophagy

• Published in: Bioscience, biotechnology, and biochemistry Vol. 84; no. 10; pp. 2062 - 2068
• Main Authors: Liu, Jingjing, Liu, Dan
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.10.2020; Oxford University Press
• Subjects: apoptosis; Apoptosis - drug effects; autophagy; Autophagy - drug effects; Cell Line; human retinal pigment epithelium cells; Humans; inflammation; Inflammation - chemically induced; Inflammation - metabolism; Lipopolysaccharides - pharmacology; Oxidative Stress - drug effects; Retinal Pigment Epithelium - cytology; Sirtuin 6; Sirtuins - metabolism; apoptosis; autophagy; inflammation; Sirtuin 6; human retinal pigment epithelium cells
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2020.1788377

Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on LPS-induced retinal injury. ARPE-19 cells were incubated with LPS to induce inflammation. The cell viability was determined using CCK-8 assay. The mRNA level and protein expression of corresponding genes was detected using qRT-PCR and western blot, respectively. The production of inflammatory cytokines was measured using ELISA kit. The levels of oxidative stress-related factors were measured using their detection kits. Cell apoptosis was observed using TUNEL assay. The results showed that Sirt6 was downregulated after LPS treatment. Sirt6 strengthened LPS-induced autophagy by promoting the expression of LC3II/I, beclin1 and ATG5. Sirt6 treatment significantly inhibited LPS-induced inflammation, oxidative stress and cell apoptosis, which was then partly abolished by 3 MA. These results suggest Sirt6 to be an important regulator for LPS-induced inflammation, oxidative stress, and apoptosis partly by regulating cell autophagy. A schematic model of the role and potential mechanism of Sirt6 in LPS-induced retinal 20 diseases.