Relationships among starch biosynthesizing protein content, fine structure and functionality in rice

• Published in: Carbohydrate polymers Vol. 237; p. 116118
• Main Authors: Bao, Jinsong, Ying, Yining, Zhou, Xin, Xu, Yanjie, Wu, Peng, Xu, Feifei, Pang, Yuehan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2020
• Subjects: Amylopectin; Biosynthesis; Resistant starch; Rice; Starch; Structure; RS; BD; Rh, AP; BE; DBE; PV; CPV; Biosynthesis; Rh, AM; HPV; SB; Tc; Rh; To; Tp; Structure; COH; SP; Rice; SS; AAC; DP (X); Starch; Amylopectin; PUL; h; GBSSI; Resistant starch; AM; CEQ; AP; SEC; ΔHr; TAC; CLD; HD; RVA; FACE; ΔHg
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.116118

[Display omitted] •Residual starch biosynthesis protein levels-structure-function relations of rice starch have been revealed.•GBSSI and SSIIIa affected amylose structure and were correlated with amylose and resistant starch content, gel texture and retrogradation.•GBSSI also affected average chain length distribution of amylopectin and was associated with pasting, thermal and retrogradation properties.•SSI, SSIIa and SSIIIa affected amylose size and were correlated with resistant starch content, thermal and gel textural properties.•SSIIa and PUL affected amylopectin peak positions and were correlated with the onset gelatinization temperature. The underlying mechanisms behind the genetics, structure and functionality relations in starches from various origins have not been fully understood. For better control of the genotypic background, rice starches from four chalky mutants and their parent were employed to investigate the above relations, and some interesting relations were revealed. GBSSI and SSIIIa were shown to affect the height of amylose in the debranched starch fraction (hAM) which reflects true amylose content (TAC), and then hAM was correlated with the AAC, RS, HD, COH, To, ΔHg, ΔHr, and R%. GBSSI also affected the average chain length (X)¯ of amylopectin, which was associated with the AAC, TAC, RS, HPV, HD, COH, ΔHg, ΔHr, and R%. The SSI, SSIIa and SSIIIa affected the amylose size (Rh,AM), which was correlated with the TAC, AAC, RS, HD, COH, To and Tp. Furthermore, both SSIIa and PUL affected the XAP2, and XAP2 was correlated with the To.