A LAMP-Based Toolbox Developed for Detecting the Major Pathogens Affecting the Production and Quality of the Chinese Medicinal Crop Aconitum carmichaelii

• Published in: Plant disease Vol. 107; no. 3; p. 658
• Main Authors: Liu, Jingzhe, Liang, Mengyi, Lin, Tao, Zhao, Qing, Wang, Huiqin, Yang, Shunyuan, Guo, Qian, Wang, Xinyi, Guo, Hua, Cui, Langjun, Yan, Yaping, Hieno, Ayaka, Kageyama, Koji, Suga, Haruhisa, Li, Mingzhu
• Format: Journal Article
• Language: English
• Published: United States 01.03.2023
• Subjects: Aconitum - microbiology; Aconitum carmichaelii; Fusarium oxysporum; F. solani; Sclerotium rolfsii; loop-mediated isothermal amplification (LAMP); Mucor circinelloides
• ISSN: 0191-2917
• DOI: 10.1094/PDIS-05-22-1092-RE

Debeaux is a traditional Chinese medicinal herb that has been utilized for approximately 2,000 years. However, as cultivation has increased, there have been more reports of infections caused by four major pathogenic fungal species, , , , and , resulting in increased disease incidences and limited production and quality. To detect these infections, we developed a LAMP-based toolbox in this study. The cytochrome c oxidase subunit 1 ( ) gene, translation elongation factor-1α ( ), internal transcribed spacer (ITS) regions of rDNA, and alcohol dehydrogenase 1 ( ) gene, respectively, were used to design species-specific LAMP primer sets for , , , and . The results showed that the LAMP-based toolbox was effective at detecting pathogens in soil and plant materials. We also used this toolbox to investigate pathogen infection in the main planting regions of . Before harvesting, , , and were commonly found in the planting fields and in infected plants. Therefore, the toolbox we developed will be useful for tracking these infections, as well as for disease control in .