Influence of chlorobenzoic acids on the growth and degradation potentials of PCB-degrading microorganisms

• Published in: World journal of microbiology & biotechnology Vol. 24; no. 7; pp. 1203 - 1208
• Main Authors: Adebusoye, Sunday Adekunle, Picardal, Flynn W, Ilori, Matthew O, Amund, Olukayode O
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.07.2008; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Acids; Applied Microbiology; Bacteria; Biochemistry; Bioconversions. Hemisynthesis; Biodegradation; Biological and medical sciences; Biological treatment; Biomedical and Life Sciences; Biotechnology; Biotransformation; Chloride; Enterobacter; Environmental Engineering/Biotechnology; Enzymes; Fundamental and applied biological sciences. Psychology; Investigations; Life Sciences; Liquid chromatography; Metabolites; Methods. Procedures. Technologies; Microbiology; Microorganisms; Original Paper; PCB; Pollutants; Polychlorinated biphenyls; Pseudomonas aeruginosa; Ralstonia; Studies; Biodegradation; Chlorobenzoic acid; Inhibition; Polychlorinated biphenyl; Biotransformation; Pollutant; Growth; Polychlorobiphenyls; Microorganism
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-007-9594-1

The biodegradation of polychlorinated biphenyls (PCBs) by diverse bacteria including those utilized in this study is often incomplete, a concomitant accumulation of chlorobenzoic acids (CBAs) are released as dead-end products. The build-up of these metabolites in the growth medium may result in feed-back inhibition and impede PCB biotransformation. In this investigation using GC-ECD and HPLC analyses, we confirmed that CBAs inhibit growth and PCB biodegradation potentials of five tropical bacteria namely, Pseudomonas aeruginosa SA-1, Enterobacter sp. SA-2, Ralstonia sp. SA-3, Ralstonia sp. SA-5 and Pseudomonas sp. SA-6. Among the four CBAs (2-CBA, 3-CBA, 4-CBA acids and 2,3-diCBA), 3-CBA was the strongest inhibitor followed by 4-CBA. Furthermore, we found that 3-CBA heavily inhibited growth of SA-3 and SA-6 on monochlorobiphenyls by 82-90% while elimination rate was inhibited by 71-88%. In the case of 2,3-diCBA, inhibition was generally less than 60%. However, effects of both acids were stronger in SA-3 than SA-6. We also found that 3-CBA and 2,3-diCBA completely inhibited carbon-chloride cleavage of 2-CB and 3-CB since cultivation in the absence of the acids resulted in recovery of 23-50% chloride in the culture fluids of organisms. These findings may therefore, have practical and ecological significance and are useful for improving the efficiency and the stability of some biological treatment processes.