Enhanced in-out-tube solid-phase microextraction by molecularly imprinted polymers-coated capillary followed by HPLC for Endocrine Disrupting Chemicals analysis
A novel Estrone (E1) molecularly imprinted polymers (MIPs-E1)-coated solid-phase microextraction (SPME) fiber was prepared by in-situ polymerization on inner and outer surface of the capillary, namely MIPs-E1-coated in-out-tube SPME (MIPs-E1-IOT-SPME), which combines the advantages of enhanced extra...
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Published in | Talanta (Oxford) Vol. 194; pp. 7 - 13 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.03.2019
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Subjects | |
Online Access | Get full text |
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Summary: | A novel Estrone (E1) molecularly imprinted polymers (MIPs-E1)-coated solid-phase microextraction (SPME) fiber was prepared by in-situ polymerization on inner and outer surface of the capillary, namely MIPs-E1-coated in-out-tube SPME (MIPs-E1-IOT-SPME), which combines the advantages of enhanced extraction efficiencies of IOT-SPME and specific adsorption abilities of MIPs, and shows nearly double enrichment capacity compared to in-tube SPME (IT-SPME). The extraction conditions were optimized and a method for determination six endocrine disrupting compounds (EDCs) by MIPs-E1-IOT-SPME coupled with HPLC is developed. The MIPs-E1 coating was characterized by fourier-transform infrared spectrometry (FTIR), scanning electron microscopy (SEM), N2 adsorption-desorption and Thermal gravimetric analysis (TGA). Under the optimum condition, the MIPs-IOT-SPME-HPLC method shows low limits of detection (LODs) (0.21–0.80 μg L−1) and limits of quantification detection (LOQs) (0.69–2.6 μg L−1), wide linear ranges from 10.0 to 1000 μg L−1 for each compound with exception of E2 and E3 from 20.0 to 1000 μg L−1, and acceptable reproducibility of one fiber for five parallel determinations is 2.4–6.6% (RSD) and fiber-to-fiber is 4.5–8.1% (RSD). The satisfactory recoveries are in the range of 88.5–105.1% with good RSDs less than 7.9% at the five spiked levels of 20.0, 50.0, 80.0, 200.0 and 500.0 μg L−1. Moreover, when compared with commercial fibers, the MIPs-E1-IOT-SPME fiber displays excellent adsorption capacity and specific selectivity.
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•An Estrone (E1) molecularly imprinted polymers-coated solid-phase microextraction fiber was prepared.•The novel SPME fiber was constructed by in-situ polymerization on inner and outer surface of the capillary.•The homemade SPME fiber shows double enrichment capacity and excellent specific selectivity compared to commercial fibers. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0039-9140 1873-3573 |
DOI: | 10.1016/j.talanta.2018.10.027 |