Folate transport in ileal brush border-membrane vesicles following extensive resection of proximal and middle small intestine in the rat

• Published in: The American journal of clinical nutrition Vol. 47; no. 1; pp. 75 - 79
• Main Authors: Said, HM, Redha, R, Tipton, W, Nylander, W
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.01.1988; American Society for Clinical Nutrition
• Subjects: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology; Animals; Biological and medical sciences; Biological Transport - drug effects; Dose-Response Relationship, Drug; folic acid; Folic Acid - metabolism; ileum; Ileum - drug effects; Ileum - metabolism; Intestine, Small - physiology; Intestine, Small - surgery; Male; Medical sciences; Methotrexate - pharmacology; Microvilli - drug effects; Microvilli - metabolism; Postoperative Period; Rats; Rats, Inbred Strains; small intestine; Stomach, duodenum, intestine, rectum, anus; Sulfasalazine - pharmacology; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; Tetrahydrofolates - pharmacology; Rat; Extensive resection; Rodentia; Vitamin; Metabolism; Small intestine; Folic acid; Vertebrata; Brush border; Mammalia; Digestive diseases; Transport; Experimental surgery
• ISSN: 0002-9165; 1938-3207
• DOI: 10.1093/ajcn/47.1.75

Uptake of folic acid (PteGlu) was examined in remnant ileum of rats after resection of 65% of the small intestine with the brush border-membrane vesicle technique. The results were compared to that of sham-operated rats. In both rat groups transport of PteGlu was linear for approximately 40 s of incubation and was similar in the presence of a Na+ and a K+ gradient (out greater than in). In resected rats transport of PteGlu was inhibited by the structural analogues 5-methyltetrahydrofolate (5-CH3H4PteGlu) and methotrexate (MTX), by sulfasalazine, and by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and was saturable as a function of concentration (apparent Kt = 18.3 microM). In the ileum of sham-operated rats, on the other hand, transport of PteGlu was not affected by 5-CH3H4PteGlu, MTX, sulfasalazine, or DIDS and was linear with concentration. These results suggest that the PteGlu transport system is induced in remnant ileum of the rat after extensive intestinal resection.