Two novel genes rapidly and transiently activated in suspension-cultured rice [Oryza sativa] cells by treatment with N-acetylchitoheptaose, a biotic elicitor for phytoalexin production

• Published in: Plant and cell physiology Vol. 37; no. 4; pp. 563 - 567
• Main Authors: Minami, E. (National Inst. of Agrobiological Resources, Tsukuba, Ibaraki (Japan)), Kuchitsu, K, He, D.Y, Kouchi, H, Midoh, N, Ohtsuki, Y, Shibuya, N
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.1996
• Subjects: Amino Acid Sequence; Biological and medical sciences; BIOLOGICAL DEVELOPMENT; CELL CULTURE; COMPOSE ORGANIQUE; COMPUESTOS ORGANICOS; CULTIVO DE CELULAS; CULTURE DE CELLULE; CULTURE TECHNIQUES; DESARROLLO BIOLOGICO; DEVELOPPEMENT BIOLOGIQUE; DNA, Complementary - genetics; DNA, Plant - genetics; Elicitor; EXPRESION GENICA; EXPRESSION DES GENES; FITOALEXINA; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Gene Expression Regulation, Plant - drug effects; Generalities. Disease free stocks; Genes, Plant; Genes. Genome; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; N-acetylchitooligosaccharide; Oligosaccharides - pharmacology; ORGANIC COMPOUNDS; Oryza - drug effects; Oryza - genetics; Oryza - metabolism; ORYZA SATIVA; PHYTOALEXINE; PHYTOALEXINS; Phytopathology. Animal pests. Plant and forest protection; Plant Extracts - biosynthesis; Rice cells; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sesquiterpenes; TECHNIQUE DE CULTURE; TECNICAS DE CULTIVO; Terpenes; Cell culture; Monocotyledones; Nucleotide sequence; Biosynthesis; Cereal crop; Characterization; Oryza sativa; Complementary DNA; Gramineae; Phytoalexin; Basic protein; Angiospermae; Isolation; Spermatophyta; Elicitation; Aminoacid sequence
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a028981

By using subtracted probes, two cDNA clones of rice, EL2 and EL3, were isolated as genes responsive within 6 min to N-acetylchitoheptaose, a potent biotic elicitor for phytoalexin biosynthesis. Analyses of the sequence of the cDNAs showed that both of EL2 and EL3 encoded basic proteins with no significant similarities to those of known genes.