Simultaneous determination of borneol and its metabolite in rat plasma by GC-MS and its application to pharmacokinetic study

• Published in: Journal of pharmaceutical analysis Vol. 4; no. 5; pp. 345 - 350
• Main Authors: Sun, Xiu-Man, Liao, Qiong-Feng, Zhou, Yu-Ting, Deng, Xue-Jiao, Xie, Zhi-Yong
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.10.2014; School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China%School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China; Xi'an Jiaotong University
• Subjects: Borneol; Camphor; GC-MS; Pharmacokinetics; Short Communication; Simultaneous determination; Wistar大鼠; 代谢产物; 同时测定; 天然冰片; 应用; 药代动力学; 血浆; Simultaneous determination; GC–MS; Pharmacokinetics; Borneol; Camphor; GC-MS
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2014.01.005

A gas chromatography mass spectrometry （GC-MS） method has been developed and fully validated for the simultaneous determination of natural borneol （NB） and its metabolite, camphor, in rat plasma. Following a single liquid-liquid extraction, the analytes were separated using an HP-5MS capillary column （0.25 mm ？ 30 m ？ 0.25μm） and analyzed by MS in the selected ion monitoring mode. Selected ion monitor （m/z） of borneol, camphor and internal standard was 95, 95 and 128, respectively. Linearity, accuracy, precision and extraction recovery of the analytes were all satisfactory. The method was successfully applied to pharmacokinetic studies of NB after oral administration to Wistar rats.