CD101 expression by Langerhans cell histiocytosis cells

• Published in: Histopathology Vol. 36; no. 3; pp. 229 - 232
• Main Authors: BOULOC, A, BOULLAND, M.-L, BAGOT, M, GEISSMANN, F, FRAITAG, S, ANDRY, P, TEILLAC, D, BENSUSSAN, A, REVUZ, J, BOUMSELL, L, WECHSLER, J
• Format: Journal Article
• Language: English
• Published: Oxford, U.K. and Cambridge, USA Blackwell Science Ltd 01.03.2000; Blackwell
• Subjects: Antigen Presentation; Antigens, CD; Antigens, Differentiation, T-Lymphocyte - immunology; Biological and medical sciences; Hematology; histiocytosis; Histiocytosis, Langerhans-Cell - immunology; Histiocytosis, Langerhans-Cell - pathology; Humans; Immunohistochemistry; Infant; Infant, Newborn; Investigative techniques, diagnostic techniques (general aspects); Langerhans cells; Medical sciences; Membrane Glycoproteins - biosynthesis; Membrane Glycoproteins - immunology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Human; Performance evaluation; Immunohistochemistry; Pathology; Langerhans cell histiocytosis; Hemopathy; Monoclonal antibody; Diagnosis; Technique; Child
• ISSN: 0309-0167; 1365-2559
• DOI: 10.1046/j.1365-2559.2000.00827.x

Aims Our objective was to study the expression of a recently identified cell surface molecule, CD101 and in Langerhans cell histiocytosis (LCH) patients as CD101 has been shown to be present on dendritic cells. We wanted to determine if CD101 expression could be helpful for the diagnosis of LCH in conjunction with other markers (CD1a, S100 protein), and could be predictive of the evolution and dissemination of the disease. Methods and results The expression of CD101 was studied by immunohistochemical technique in 11 cases of Langerhans cell histiocytosis on frozen sections. The expression of CD101 was positive in nine cases, high in six cases and low in three cases. There was no expression in the other two cases. No correlation with the evolution, the localization or the dissemination of the disease could be evidenced. Conclusions CD101 is a new phenotypic marker that might be useful in combination with other markers for the diagnosis of LCH. However, as the anti‐CD101 antibody works only in frozen sections, its value is limited compared to anti‐CD1a antibody.