Macrophage Colony‐Stimulating Factor (M‐CSF), As Well As Granulocyte Colony‐Stimulating Factor (G‐CSF), Accelerates Neovascularization

• Published in: Stem cells (Dayton, Ohio) Vol. 23; no. 3; pp. 347 - 354
• Main Authors: Minamino, Keizo, Adachi, Yasushi, Okigaki, Mitsuhiko, Ito, Hidefumi, Togawa, Yoshimi, Fujitha, Kengo, Tomita, Minoru, Suzuki, Yasuhiro, Zhang, Yuming, Iwasaki, Masayoshi, Nakano, Keiji, Koike, Yasushi, Matsubara, Hiroaki, Iwasaka, Toshiji, Matsumura, Miyo, Ikehara, Susumu
• Format: Journal Article
• Language: English
• Published: Bristol John Wiley & Sons, Ltd 01.03.2005
• Subjects: Animals; Bone marrow cell; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Bone Marrow Transplantation; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Endothelium - chemistry; Granulocyte Colony-Stimulating Factor - pharmacology; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; G‐CSF; H-2 Antigens - analysis; Hindlimb - blood supply; Hindlimb - drug effects; Hindlimb - pathology; Ischemia - physiopathology; Macrophage Colony-Stimulating Factor - pharmacology; Mesenchymal Stromal Cells - cytology; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Transgenic; Muscle, Skeletal - blood supply; M‐CSF; Neovascularization; Neovascularization, Physiologic - drug effects; Regional Blood Flow - drug effects; Vascular Endothelial Growth Factor A - metabolism
• ISSN: 1066-5099; 1549-4918
• DOI: 10.1634/stemcells.2004-0190

It has been reported that bone marrow cells (BMCs) differentiate into endothelial cells of blood vessels, and that granulocyte colony‐stimulating factor (G‐CSF) mobilizes progenitors in the BMCs to the peripheral blood, while macrophage colony‐stimulating factor (M‐CSF) augments the production of monocytes. We examined whether M‐CSF augments the differentiation of BMCs into endothelial cells of blood vessels using a hindlimb‐ischemic model. Either G‐CSF or M‐CSF, or both, was administered to the hindlimb‐ischemic mice for 3 days. Both M‐CSF and G‐CSF augmented the differentiation of BMCs into endothelial cells of blood vessels through vascular endothelial cell growth factor (VEGF), resulting in early recovery of blood flow in the ischemic limbs.