Development of first linkage map for Silphium integrifolium (Asteraceae) enables identification of sporophytic self-incompatibility locus

Silphium integrifolium (Asteraceae) has been identified as a candidate for domestication as a perennial oilseed crop and is assumed to have sporophytic self-incompatibility system-the genetic basis of which is not well understood in the Asteraceae. To address this gap, we sought to map the genomic l...

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Bibliographic Details
Published inHeredity Vol. 128; no. 5; pp. 304 - 312
Main Authors Price, John H, Raduski, Andrew R, Brandvain, Yaniv, Van Tassel, David L, Smith, Kevin P
Format Journal Article
LanguageEnglish
Published England Springer Nature B.V 01.05.2022
Springer International Publishing
Subjects
Asteraceae
Asteraceae - genetics
Chromosome Mapping
Domestication
Gene mapping
Genetic Linkage
Genetics
Genomes
Genomics
Genotype & phenotype
Genotypes
Genotyping
Helianthus annuus
Humans
Incompatibility
Lactuca sativa
Loci
Oilseed crops
Oilseeds
Population
Self-incompatibility
Self-recognition
Siblings
Silphium integrifolium
Single-nucleotide polymorphism
Synteny
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Summary:Silphium integrifolium (Asteraceae) has been identified as a candidate for domestication as a perennial oilseed crop and is assumed to have sporophytic self-incompatibility system-the genetic basis of which is not well understood in the Asteraceae. To address this gap, we sought to map the genomic location of the self-recognition locus (S-locus) in this species. We used a biparental population and genotyping-by-sequencing to create the first genetic linkage map for this species, which contained 198 SNP markers and resolved into the correct number of linkage groups. Then we developed a novel crossing scheme and set of analysis methods in order to infer S-locus genotypes for a subset of these individuals, allowing us to map the trait. Finally, we evaluated potential genes of interest using synteny analysis with the annual sunflower (Helianthus annuus) and lettuce (Lactuca sativa) genomes. Our results confirm that S. integrifolium does indeed have a sporophytic self-incompatibility system. Our method is effective and efficient, allowed us to map the S. integrifolium S-locus using fewer resources than existing methods, and could be readily applied to other species.
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ISSN:0018-067X
1365-2540
DOI:10.1038/s41437-022-00530-4