Comparative study of horseshoe crab tropomyosin

1. 1. Immunological and biochemical properties of skeletal muscle tropomyosin were investigated to elucidate phylogenetic relationships among four extant species of horseshoe crabs, Tachypleus tridentatus, T. gigas, Carcinoscorpius rotundicauda, and Limulus polyphemus. 2. 2. Immunodiffusion tests us...

Full description

Saved in:
Bibliographic Details
Published inComparative biochemistry and physiology. B, Comparative biochemistry Vol. 93; no. 3; pp. 681 - 687
Main Authors Miyazaki, Jun-Ichi, Ishimoda-Takagi, Tadashi, Sekiguchi, Koichi, Hirabayashi, Tamio
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 1989
Elsevier Science
Subjects
Biological and medical sciences
Carcinoscorpius rotundicauda
Fundamental and applied biological sciences. Psychology
Invertebrates
Less representative or incertae sedis groups: acanthocephala, chaetognatha, gnathostomulida, lophophoria, merostomata, mesozoa, myxozoa, nematorhyncha, pararthropoda, placozoa, priapuloidea, prochordata, pycnogonida, rotifera
Limulus polyphemus
Marine
Tachypleus gigas
Tachypleus tridentatus
Xiphosura
Gel electrophoresis
Tropomyosin
Interspecific comparison
Contractile protein
Phylogeny
Striated muscle
Immunological method
Marine environment
Antigenicity
Arthropoda
Electrophoretic mobility
Merostomata
Two dimensional electrophoresis
Invertebrata
Limulus polyphemus
Online AccessGet full text

Cover

More Information
Summary:1. 1. Immunological and biochemical properties of skeletal muscle tropomyosin were investigated to elucidate phylogenetic relationships among four extant species of horseshoe crabs, Tachypleus tridentatus, T. gigas, Carcinoscorpius rotundicauda, and Limulus polyphemus. 2. 2. Immunodiffusion tests using an antiserum against tropomyosin from T. tridentatus showed no difference of antigenicities among the four species. Peptide patterns of enzymatically or chemically cleaved tropomyosin also represented no significant difference. 3. 3. However, electrophoretic mobilities of the proteins were found to be significantly different between L. polyphemus and three other species when long distance electrophoresis was conducted. 4. 4. The mobility difference revealed a distinctive property of L. polyphemus tropomyosin and confirmed that the species is phylogenetically differentiated far from the other species.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-0491
DOI:10.1016/0305-0491(89)90395-7