Streptococcus lactarius sp. nov., isolated from breast milk of healthy women

Three strains of a hitherto-unknown, Gram-stain-positive coccus were recovered from the milk of three non-related healthy women. The isolates shared 99 % 16S rRNA gene sequence similarity with sequences from uncultured members of the Lactobacillales and Streptococcus. The closest sequence correspond...

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Published inInternational journal of systematic and evolutionary microbiology Vol. 61; no. Pt 5; pp. 1048 - 1052
Main Authors MARTIN, Virginia, MANES-LAZARO, Rosario, RODRIGUEZ, Juan Miguel, MALDONADO-BARRAGAN, Antonio
Format Journal Article
LanguageEnglish
Published Reading Society for General Microbiology 01.05.2011
Subjects
Bacteriology
Biological and medical sciences
DNA, Bacterial - genetics
Female
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Milk, Human - microbiology
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S - genetics
Streptococcus - classification
Streptococcus - genetics
Streptococcus - isolation & purification
Streptococcus - metabolism
Systematics
Human
Taxonomy
Basidiomycota
Fungi
Streptococcaceae
Streptococcus
Bacteria
Micrococcales
Female
New species
Lactarius
Woman
Breast milk
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Summary:Three strains of a hitherto-unknown, Gram-stain-positive coccus were recovered from the milk of three non-related healthy women. The isolates shared 99 % 16S rRNA gene sequence similarity with sequences from uncultured members of the Lactobacillales and Streptococcus. The closest sequence corresponding to a defined species was that of Streptococcus peroris GTC 848(T), with a similarity of 98 %. A partial sequence (488 bp) of the tuf gene also showed 97 % similarity with that of S. peroris CCUG 39814(T). The combined 16S rRNA/tuf-based phylogeny revealed that all the isolates grouped in a statistically well-supported cluster separate from S. peroris. Enzyme activity profiles as well as fermentation patterns differentiated the novel bacteria from other members of the Streptococcus mitis group. Finally, phenotypic, genotypic and phylogenetic data supported the proposal of a novel species of the genus Streptococcus, for which the name Streptococcus lactarius sp. nov. is proposed. The type strain is MV1(T) ( = CECT 7613(T)  = DSM 23027(T)).
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ISSN:1466-5026
1466-5034
DOI:10.1099/ijs.0.021642-0