A human gastric cancer cell line possesses a functional receptor for gastrin-releasing peptide

Bombesin (BBS) exhibits diverse biological functions including those of neurotransmitter, regulator of gastrointestinal hormone release, and mitogen. Gastrin-releasing peptide (GRP, the mammalian equivalent of BBS) is found in mucosal cells of the gastric fundus and antrum. We determined whether a h...

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Bibliographic Details
Published inCancer investigation Vol. 16; no. 1; p. 12
Main Authors Bold, R J, Kim, H J, Ishizuka, J, Townsend, Jr, C M, Thompson, J C
Format Journal Article
LanguageEnglish
Published England 1998
Subjects
Adenocarcinoma - pathology
Bombesin - metabolism
Calcium - metabolism
Cross-Linking Reagents
DNA, Complementary - genetics
Gene Expression
Humans
Receptors, Bombesin - metabolism
RNA, Messenger - genetics
Stomach Neoplasms - pathology
Tumor Cells, Cultured - pathology
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Summary:Bombesin (BBS) exhibits diverse biological functions including those of neurotransmitter, regulator of gastrointestinal hormone release, and mitogen. Gastrin-releasing peptide (GRP, the mammalian equivalent of BBS) is found in mucosal cells of the gastric fundus and antrum. We determined whether a human gastric cancer cell line (SIIA) expresses a functional GRP-receptor (GRP-R). BBS increased intracellular calcium ([Ca2+]i), and a specific GRP-R antagonist, ([D-Phe6, Des-Met14]-BBS (6-14)-ethylamide), blocked BBS-induced increase in [Ca2+]i. SIIA cells possess GRP-R mRNA by reverse transcriptase-PCR. Furthermore, these cells possess an 80-kDa cell surface protein that specifically binds BBS with two high-binding affinities (Kd1 = 0.6 nM, Kd2 = 6.7 nM). These findings indicate that SIIA cells possess a GRP-R that is capable of physiological signal transduction, though the cellular response remains unknown.
ISSN:0735-7907
DOI:10.3109/07357909809039748