High-Throughput Automated Microscopy of Circulating Tumor Cells

• Published in: Scientific reports Vol. 9; no. 1; pp. 13766 - 9
• Main Authors: Aguilar-Avelar, Carlos, Soto-García, Brenda, Aráiz-Hernández, Diana, Yee-de León, Juan F, Esparza, Miguel, Chacón, Franco, Delgado-Balderas, Jesús Rolando, Alvarez, Mario M, Trujillo-de Santiago, Grissel, Gómez-Guerra, Lauro S, Velarde-Calvillo, Liza P, Abarca-Blanco, Alejandro, Wong-Campos, J D
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 24.09.2019; Nature Publishing Group UK
• Subjects: Automation; Biomarkers, Tumor - metabolism; Cancer; Cell Count - methods; Cell Line, Tumor; Cell Separation - methods; Humans; Microscopy; Microscopy, Fluorescence - methods; Neoplastic Cells, Circulating - metabolism; Neoplastic Cells, Circulating - pathology; Prognosis; Tumor cells
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-50241-w

Circulating tumor cells (CTCs) have the potential of becoming the gold standard marker for cancer diagnosis, prognosis and monitoring. However, current methods for its isolation and characterization suffer from equipment variability and human operator error that hinder its widespread use. Here we report the design and construction of a fully automated high-throughput fluorescence microscope that enables the imaging and classification of cancer cells that were labeled by immunostaining procedures. An excellent agreement between our machine vision-based approach and a state-of-the-art microscopy equipment was achieved. Our integral approach provides a path for operator-free and robust analysis of cancer cells as a standard clinical practice.