Physical mapping of the Magnaporthe grisea AVR1-MARA locus reveals the virulent allele contains two deletions

The avirulence genes that have been identified in Magnaporthe grisea exhibit varying degrees of stability in infection assays. AVR1-MARA is considered one of the stable avirulence genes. In an effort to understand this stability, we analyzed the AVR1-MARA locus by physical mapping and chromosome wal...

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Published inMolecular plant-microbe interactions Vol. 10; no. 9; pp. 1102 - 1105
Main Authors Mandel, M.A, Crouch, V.W, Gunawardena, U.P, Harper, T.M, Orbach, M.J
Format Journal Article
LanguageEnglish
Published St Paul, MN APS Press 01.12.1997
The American Phytopathological Society
Subjects
Biological and medical sciences
CARTE GENETIQUE
CHROMOSOME MANIPULATION
CHROMOSOME MAPS
CHROMOSOME WALKING
DESCENDANCE
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
GENE
GENES
GENETIC MAPPING
GENETIC MAPS
GENETIC MARKERS
LOCI
LOCUS
MAGNAPORTHE
MANIPULACION DE CROMOSOMAS
MANIPULATION CHROMOSOMIQUE
MAPAS GENETICOS
MARCADORES GENETICOS
MARQUEUR GENETIQUE
MUTANT
MUTANTES
MUTANTS
NUCLEOTIDE SEQUENCE
PATHOGENICITY
PATOGENICIDAD
Phytopathology. Animal pests. Plant and forest protection
POUVOIR PATHOGENE
PROGENIE
PROGENY
RFLP
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Variation, races, biotypes, parasitic specialization, genetics
VIRULENCE
Fungi
Plant pathogen
Gene
Virulence
Deletion
Ascomycetes
Mutation
Thallophyta
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Summary:The avirulence genes that have been identified in Magnaporthe grisea exhibit varying degrees of stability in infection assays. AVR1-MARA is considered one of the stable avirulence genes. In an effort to understand this stability, we analyzed the AVR1-MARA locus by physical mapping and chromosome walking. By walking toward AVR1-MARA from restriction fragment length polymorphism markers on both sides of the locus, we isolated sequences that are inseparable from AVR1-MARA, but we were unable to clone the complete locus. In contrast, the virulent locus avr1-MARA was isolated easily. A detailed comparative map of the two loci was constructed that identifies two deletions in the virulent locus, suggesting that virulence may be due to partial or complete deletion of the gene. Physical mapping also revealed that one progeny strain from a cross between avirulent and virulent parents appears to have spontaneously mutated to virulence
Bibliography:1997070757
F30
H20
ISSN:0894-0282
1943-7706
DOI:10.1094/mpmi.1997.10.9.1102