Radioimmunoassay of haemoglobin F in K 562 cells following induction with renin substrate and erythropoietin

To test the hypothesis of renin substrate (RS; angiotensinogen) being a precursor of erythropoietin (EP), the capacity of RS and EP to induce Hb synthesis was compared in cultured human erythroid leukaemia cells of the K 562 line after prestimulation with haemin. For this purpose a radioimmunoassay...

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Published inScandinavian journal of clinical and laboratory investigation Vol. 45; no. 4; p. 373
Main Authors Rosenlöf, K, Fyhrquist, F, Hortling, L, Grönhagen-Riska, C
Format Journal Article
LanguageEnglish
Published England 01.01.1985
Subjects
Angiotensinogen - pharmacology
Angiotensins - pharmacology
Cell Line
Erythropoietin - pharmacology
Fetal Hemoglobin - analysis
Humans
Leukemia - blood
Radioimmunoassay
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Summary:To test the hypothesis of renin substrate (RS; angiotensinogen) being a precursor of erythropoietin (EP), the capacity of RS and EP to induce Hb synthesis was compared in cultured human erythroid leukaemia cells of the K 562 line after prestimulation with haemin. For this purpose a radioimmunoassay for haemoglobin F (HbF) was developed. This assay was shown to be specific for HbF, reproducible, and sensitive for 0.1 ng of HbF. The cells were induced by RS and EP to increased HbF production. Cells stimulated with RS or EP showed increased benzidine staining. This data, corroborating our earlier observations on immunological similarities between RS and EP, supports the hypothesis that renin substrate is a likely precursor of erythropoietin.
ISSN:0036-5513
1502-7686
DOI:10.3109/00365518509161021