Adsorption of proteins at physiological concentrations on pegylated surfaces and the compatibilizing role of adsorbed albumin with respect to other proteins according to optical waveguide lightmode spectroscopy (OWLS)

• Published in: Journal of biomaterials science. Polymer ed. Vol. 24; no. 13; pp. 1499 - 1518
• Main Authors: Leclercq, Laurent, Modena, Enrico, Vert, Michel
• Format: Journal Article
• Language: English
• Published: England Routledge 01.09.2013; Taylor & Francis
• Subjects: Adsorption; albumin; antifouling; Blood Proteins - chemistry; Chemical Sciences; Coated Materials, Biocompatible - chemistry; fibrinogen; Fibrinogen - chemistry; gamma-Globulins - chemistry; Humans; Lactates - chemistry; Lactic Acid - chemistry; Materials Testing; optical waveguide lightmode spectroscopy; Organic chemistry; poly(lactic acid)-block-poly(ethylene oxide); Polyesters; Polyethylene Glycols - chemistry; Polymers - chemistry; protein adhesion; Serum Albumin - chemistry; Silicon Dioxide - chemistry; Spectrum Analysis - instrumentation; Spectrum Analysis - methods; Surface Properties; Titanium - chemistry; γ-globulins; optical waveguide lightmode spectroscopy; γ\-globulins; fibrinogen; albumin; protein adhesion; antifouling; poly\(lactic acid\)\-block\-poly\(ethylene oxide\)
• ISSN: 0920-5063; 1568-5624
• DOI: 10.1080/09205063.2013.772045

In literature, contacts between pegylated compounds and blood proteins are generally discussed in terms of excluded volume-related repulsions although adsorption and compatibility have been reported for some of these proteins occasionally. The major problem to investigate the behavior of blood in contact with pegylated surfaces is the complexity of the medium and especially the presence of albumin in large excess. In a model approach, optical waveguide lightmode spectroscopy (OWLS) was used to monitor the fate of albumin, fibrinogen, and γ-globulins at physiological concentrations in pH = 7.4 isotonic HEPES buffer after contact with SiTiO 2 chips coated with diblock poly(DL-lactic acid)-block-poly(ethylene oxide)s and triblock poly(DL-lactic acid)-block-poly(ethylene oxide)-block-poly(DL-lactic acid) copolymers. Corresponding homopolymers were used as controls. The three protein systems were investigated separately, as a mixture and when added successively according to different orders of addition. OWLS gave access to the mass and the thickness of adhering protein layers that resist washing with HEPES buffer. Protein depositions were detected regardless of the presence of poly(ethylene glycol) segments on surfaces. Adsorption depended on the protein, on the surface and also on the presence of the other proteins. Unexpectedly any surface coated with a layer of adsorbed albumin prevented deposition of other proteins, including albumin itself. This outstanding finding suggests that it was the presence of albumin adsorbed on a surface, pegylated or not, that made that surface compatible with other proteins. As a consequence, dipping a device to be in contact with the blood of a patient in a solution of albumin could be a very simple means to avoid further protein deposition and maybe platelets adhesion after in vivo implantation.