Silencing of CD86 in dendritic cells by small interfering RNA regulates cytokine production in T cells from patients with allergic rhinitis in vitro

• Published in: Molecular medicine reports Vol. 20; no. 4; pp. 3893 - 3900
• Main Authors: Sun, Rong, Yang, Yang, Gu, Zheng, Tang, Xinye, Zhang, Cheng, Kou, Wei, Wei, Ping
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.10.2019; Spandidos Publications UK Ltd
• Subjects: Allergic rhinitis; Allergies; Antigens; Antigens, CD - genetics; Antigens, Differentiation, Myelomonocytic - genetics; Asthma; Bone morphogenetic proteins; CD80 antigen; CD86 antigen; Cell activation; Cells, Cultured; Coculture Techniques; Comparative analysis; Cytokines; Cytokines - genetics; Dendritic cells; Dendritic Cells - metabolism; Down-Regulation; Experiments; Humans; Hypersensitivity; Infection; Inflammation; Interleukin 5; Interleukins; Kinases; Lymphocytes; Lymphocytes T; Neomycin; Peripheral blood; Proteins; Rhinitis; Rhinitis, Allergic - genetics; RNA; RNA Interference; RNA, Small Interfering - genetics; Scientific equipment industry; siRNA; Studies; T cells; T-Lymphocytes - metabolism; Transfection; Up-Regulation; United States
• ISSN: 1791-2997; 1791-3004; 1791-3004
• DOI: 10.3892/mmr.2019.10638

The aim of the present study was to investigate the expression and role of the co‑stimulatory molecule T‑lymphocyte activation antigen CD86 (CD86) in dendritic cells (DCs) from the peripheral blood of patients with allergic rhinitis (AR) compared with those from healthy individuals. It was observed that mature DCs from the peripheral blood of patients with AR expressed high levels of the co‑stimulatory molecule CD86, but not CD80, compared with healthy control subjects. CD86 expression levels in DCs decreased significantly following transfection with siRNA in a lentiviral vector. Furthermore, the level of transforming growth factor‑β1 produced by T cells co‑cultured with DCs was significantly increased in the siRNA group, while interleukin (IL)‑4 and IL‑5 production was significantly decreased. The findings of the present study indicated that CD86 may play a pivotal role in the regulatory T cell/type 2 helper T cell imbalance in allergic inflammation.