Shortening deactivation: quantifying a critical component of cyclical muscle contraction

• Published in: American Journal of Physiology: Cell Physiology Vol. 322; no. 4; pp. C653 - C665
• Main Authors: Loya, Amy K, Van Houten, Sarah K, Glasheen, Bernadette M, Swank, Douglas M
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.04.2022
• Subjects: Animals; Deactivation; Drosophila; Drosophila - physiology; Insects; Isometric Contraction - physiology; Lethocerus; Muscle contraction; Muscle Contraction - physiology; insect; power; shortening deactivation; stretch activation; locomotion; muscle mechanics
• ISSN: 0363-6143; 1522-1563; 1522-1563
• DOI: 10.1152/ajpcell.00281.2021

A muscle undergoing cyclical contractions requires fast and efficient muscle activation and relaxation to generate high power with relatively low energetic cost. To enhance activation and increase force levels during shortening, some muscle types have evolved stretch activation (SA), a delayed increased in force following rapid muscle lengthening. SA's complementary phenomenon is shortening deactivation (SD), a delayed decrease in force following muscle shortening. SD increases muscle relaxation, which decreases resistance to subsequent muscle lengthening. Although it might be just as important to cyclical power output, SD has received less investigation than SA. To enable mechanistic investigations into SD and quantitatively compare it to SA, we developed a protocol to elicit SA and SD from and indirect flight muscles (IFM) and jump muscle. When normalized to isometric tension, IFM exhibited a 118% SD tension decrease, IFM dropped by 97%, and jump muscle decreased by 37%. The same order was found for normalized SA tension: IFM increased by 233%, IFM by 76%, and jump muscle by only 11%. SD occurred slightly earlier than SA, relative to the respective length change, for both IFMs; but SD was exceedingly earlier than SA for jump muscle. Our results suggest SA and SD evolved to enable highly efficient IFM cyclical power generation and may be caused by the same mechanism. However, jump muscle SA and SD mechanisms are likely different, and may have evolved for a role other than to increase the power output of cyclical contractions.