Increase in insulin binding affinity by chloroquine in cultured rat hepatoma cells

Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed. Scatchard analysis showed that chloroquine increas...

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Bibliographic Details
Published inEndocrine research Vol. 13; no. 1; p. 49
Main Authors Sorimachi, K, Okayasu, T, Yasumura, Y
Format Journal Article
LanguageEnglish
Published England 1987
Subjects
Animals
Cell Membrane - metabolism
Cells, Cultured
Chloroquine - pharmacology
Insulin - metabolism
Kinetics
Liver Neoplasms, Experimental - metabolism
Rats
Receptor, Insulin - drug effects
Receptor, Insulin - metabolism
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Summary:Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed. Scatchard analysis showed that chloroquine increased the affinity of insulin for cells at both 4 degrees C and 23 degrees C, and that this increase eventually caused the accumulation of insulin internalized by cells at 23 degrees C.
ISSN:0743-5800
DOI:10.1080/07435808709023662