Anomeric preference in uptake of D-glucose and of D-galactose by rat lenses

To determine the anomeric preference of uptake of D-glucose and of D-galactose by rat lenses, we crystallized alpha-, beta-D-[U-14C]glucose (720 microCi/mmol) and alpha-, beta-D-[U-14C]galactose (180 microCi/mmol) by our method and incubated them separately with rat lenses for 1 min, because of the...

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Bibliographic Details
Published inCurrent eye research Vol. 6; no. 10; p. 1223
Main Authors Okuda, J, Kawamura, M, Didelot, S
Format Journal Article
LanguageEnglish
Published England 1987
Subjects
Animals
Binding, Competitive
Chemical Phenomena
Chemistry
Crystallization
Galactose - metabolism
Glucose - metabolism
Lens, Crystalline - metabolism
Male
Rats
Rats, Inbred Strains
Time Factors
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Summary:To determine the anomeric preference of uptake of D-glucose and of D-galactose by rat lenses, we crystallized alpha-, beta-D-[U-14C]glucose (720 microCi/mmol) and alpha-, beta-D-[U-14C]galactose (180 microCi/mmol) by our method and incubated them separately with rat lenses for 1 min, because of the short half-life of mutarotation of alpha-D-glucose (9.6 min) and of alpha-D-galactose (4.6 min) in HEPES medium at 30 degrees C. During aerobic incubation of rat lenses in HEPES medium containing radioactive alpha or beta anomer of D-glucose, there was no significant difference in the rate of uptake between alpha and beta anomers of D-glucose by rat lenses. However, 1.59 times greater incorporation of alpha-D-galactose was observed over that of beta-D-galactose under the same conditions.
ISSN:0271-3683
DOI:10.3109/02713688709025232