Characterization and gene mapping of a chlorophyll-deficient mutant clm1 of Triticum monococcum L

• Published in: Biologia plantarum Vol. 57; no. 3; pp. 442 - 448
• Main Authors: Ansari, M. J., Al-Ghamdi, A., Kumar, R., Usmani, S., Al-Attal, Y., Nuru, A., Mohamed, A. A., Singh, K., Dhaliwal, H. S.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2013; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: Biomedical and Life Sciences; bulk segregant analysis; diploid wheat; ethylmethane sulfonate; gene mapping; Life Sciences; Plant Sciences; ssr marker; Triticum aestivum; Triticum monococcum; bulk segregant analysis; ethylmethane sulfonate; diploid wheat; SSR marker; gene mapping
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-013-0307-3

Diploid wheat Triticum monococcum L. is a model plant for wheat functional genomics. Chlorophyll-deficient mutant ( clm1 ) was identified during manual screening of the ethyl methanesulphonate (EMS)-treated M 2 progenies of T. monococcum accession pau14087 in the field. The clm1 mutant, due to significantly decreased chlorophyll content compared with the wild-type (WT), exhibited pale yellow leaves which slowly recovered to green before flowering. The clm1 mutant showed early flowering, reduced number of tillers, trichome length and density, and different shape as compared with the WT. At the same time, clm1 mutant culm had more chlorophyll-containing parenchymatous tissues compared to WT, presumably to absorb more sunlight for photosynthesis. Genetic analysis indicated that the clm1 mutation was monogenic recessive. The clm1 mutant was mapped between Xgwm473 and Xwmc96 SSR markers, with genetic distances of 2.1 and 2.6 cM, respectively, on the 7A m L chromosome.