Evaluation of a new flow cytometry based method for detection of BCR-ABL1 fusion protein in chronic myeloid leukemia

• Published in: Blood research Vol. 52; no. 2; pp. 112 - 118
• Main Authors: Dasgupta, Swati, Ray, Ujjal K, Mitra, Arpita Ghosh, Bhattacharyya, Deboshree M, Mukhopadhyay, Ashis, Das, Priyabrata, Gangopadhyay, Sudeshna, Roy, Sudip, Mukhopadhyay, Soma
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society of Hematology; Korean Society of Blood and Marrow Transplantation; Korean Society of Pediatric Hematology-Oncology; Korean Society on Thrombosis and Hemostasis 01.06.2017; 대한혈액학회
• Subjects: Original; 병리학; Flow cytometry; Chronic myeloid leukemia; BCR-ABL1; Philadelphia chromosome
• ISSN: 2287-979X; 2288-0011
• DOI: 10.5045/br.2017.52.2.112

Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving and genes, respectively. An accurate and reliable detection of fusion gene is necessary for the diagnosis and monitoring of CML. Previously, many technologies, most of which are laborious and time consuming, have been developed to detect chimeric gene or chromosome. A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion proteins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of positivity by three routinely used procedures like karyotyping, and quantitative real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.