Genome-wide identification of members of the Skp1 family in almond (Prunus dulcis), cloning and expression characterization of PsdSSK1

• Published in: Physiology and molecular biology of plants Vol. 29; no. 1; pp. 35 - 49
• Main Authors: Zhang, Dongdong, Yu, Zhenfan, Hu, Shaobo, Liu, Xingyue, Zeng, Bin, Gao, Wenwen, Qin, HuanXue, Ma, Xintong, He, Yawen
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 2023; Springer Nature B.V
• Subjects: Amino acid sequence; Biodegradation; Biological activity; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Cell Biology; Cell cycle; Chromosomes; Cloning; Conserved sequence; Cross-pollination; Family studies; Flowers; Fluorescence; Gene expression; Gene regulation; Genes; Genomes; Incompatibility; Kinases; Life Sciences; Phylogeny; Plant Physiology; Plant Sciences; Pollen; Pollination; Proteins; Prunus dulcis; Research Article; Self-incompatibility; Signal processing; Signal transduction; Transcriptomes; Ubiquitin; Ubiquitin-protein ligase; Evolutionary analyses; gene family; Functional prediction; Expression pattern analyses; Skp1 gene family; Prunus dulcis
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-023-01278-9

Skp1 (S-phase kinase-associated protein 1) is the core gene of SCF ubiquitin ligase, which mediates protein degradation, thereby regulating biological processes such as cell cycle progression, transcriptional regulation, and signal transduction. A variety of plant Skp1 gene family studies have been reported. However, the almond Skp1 gene family has not yet been studied. In this study, we identified 18 members of the Prunus dulcis PdSkp1 family that were unevenly distributed across six chromosomes of the almond genome. Phylogenetic tree analysis revealed that the PdSkp1 members can be divided into three groups: I, II, and III. PdSkp1 members in each subfamily have relatively conserved motif types and exon/intron numbers. There were three pairs of fragment duplication genes and one pair of tandem repeat genes, and their functions were highly evolutionarily conserved. Transcriptome data showed that PdSkp1 is expressed in almond flower tissues, and that its expression shows significant change during cross-pollination. Fluorescence quantitative results showed that eight PdSkp1 genes had different expression levels in five tissues of almond, i.e., branches, leaves, flower buds, flesh, and cores. In addition, we cloned a PsdSSK1 gene based on PdSkp1 . The cloned PsdSSK1 showed the same protein sequence as PdSkp1-12 . Results of qPCR and western blot analysis showed high expression of PsdSSK1 in almond pollen. In conclusion, we report the first clone of the key gene SSK1 that controls self-incompatibility in almonds. Our research lays a foundation for future functional research on PdSkp1 members, especially for exploring the mechanism of almond self-incompatibility.