Rapid detection of CITES-listed shark fin species by loop-mediated isothermal amplification assay with potential for field use

Shark fin is a delicacy in many Asian countries. Overexploitation of sharks for shark fin trading has led to a drastic reduction in shark population. To monitor international trade of shark fin products and protect the endangered species from further population decline, we present rapid, user-friend...

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Published inScientific reports Vol. 10; no. 1; p. 4455
Main Authors But, Grace Wing-Chiu, Wu, Hoi-Yan, Shao, Kwang-Tsao, Shaw, Pang-Chui
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 10.03.2020
Nature Publishing Group UK
Subjects
Cytochrome oxidase I
Endangered species
Polymerase chain reaction
Population decline
Sharks
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Summary:Shark fin is a delicacy in many Asian countries. Overexploitation of sharks for shark fin trading has led to a drastic reduction in shark population. To monitor international trade of shark fin products and protect the endangered species from further population decline, we present rapid, user-friendly and sensitive diagnostic loop-mediated isothermal amplification (LAMP) and effective polymerase chain reaction (PCR) assays for all twelve CITES-listed shark species. Species-specific LAMP and PCR primers were designed based on cytochrome oxidase I (COI) and NADH2 regions. Our LAMP and PCR assays have been tested on 291 samples from 93 shark and related species. Target shark species could be differentiated from non-target species within three hours from DNA extraction to LAMP assay. The LAMP assay reported here is a simple and robust solution for on-site detection of CITES-listed shark species with shark fin products.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-61150-8