Pseudonocardia kunmingensis sp. nov., an actinobacterium isolated from surface-sterilized roots of Artemisia annua L

• Published in: International journal of systematic and evolutionary microbiology Vol. 61; no. Pt 9; pp. 2292 - 2297
• Main Authors: ZHAO, Guo-Zhen, JIE LI, HUANG, Hai-Yu, ZHU, Wen-Yong, PARK, Dong-Jin, KIM, Chang-Jin, XU, Li-Hua, LI, Wen-Jun
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.09.2011
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - physiology; Aerobiosis; Artemisia annua - microbiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Carbohydrates - analysis; Cell Wall - chemistry; China; Cluster Analysis; Diaminopimelic Acid - analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Plant Roots - microbiology; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; China; Root; Taxonomy; Pseudonocardiaceae; Compositae; Medicinal plant; Artemisia annua; Pseudonocardia; Dicotyledones; Angiospermae; Bacteria; Actinomycetes; Spermatophyta; New species
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.027607-0

A Gram-positive, aerobic, actinobacterial strain with rod-shaped spores, designated YIM 63158(T), was isolated from the surface-sterilized roots of Artemisia annua L. collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 63158(T) belonged to the genus Pseudonocardia. The closest neighbours were 'Pseudonocardia sichuanensis' KLBMP 1115 (99.9 % 16S rRNA gene sequence similarity), Pseudonocardia adelaidensis EUM 221(T) (99.1 %) and Pseudonocardia zijingensis DSM 44774(T) (98.8 %); sequence similarities to other members of the genus Pseudonocardia ranged from 98.6 to 94.4 %. The chemotaxonomic characteristics, such as the cell-wall diaminopimelic acid, whole-cell sugars, fatty acid components and major menaquinones, suggested that the isolate belonged to the genus Pseudonocardia. The G+C content of the genomic DNA was 73.3 mol%. On the basis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA relatedness between the isolate and other members of the genus Pseudonocardia, it is proposed that strain YIM 63158(T) represents a novel species in this genus, with the name Pseudonocardia kunmingensis sp. nov. The type strain is YIM 63158(T) ( = DSM 45301(T)  = CCTCC AA 208078(T)). [corrected].