Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study in Brassica

The potential use of RAPDs for taxonomic studies were investigated using Brassica, Sinapis and Raphanus taxa. Principal coordinate analysis of 284 RAPD bands revealed the classical U triangle relationship between diploid and amphidiploid Brassica taxa. Raphanus sativus and S. alba were distinct from...

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Bibliographic Details
Published inTheoretical and applied genetics Vol. 84; no. 7-8; p. 990
Main Authors Demeke, T. (Baylor Univ., Sandy, UT (USA).), Adams, R.P, Chibbar, R
Format Journal Article
LanguageEnglish
Published Germany 01.09.1992
Subjects
ADN
BRASSICA
CASE STUDIES
DNA
ESTUDIOS DE CASOS PRACTICOS
ETUDE DE CAS
GENETIC POLYMORPHISM
PCR
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
Primers
RAPDs
TAXONOMIA
TAXONOMIE
TAXONOMY
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Summary:The potential use of RAPDs for taxonomic studies were investigated using Brassica, Sinapis and Raphanus taxa. Principal coordinate analysis of 284 RAPD bands revealed the classical U triangle relationship between diploid and amphidiploid Brassica taxa. Raphanus sativus and S. alba were distinct from the Brassica taxa. It appears that at least ten primers with approximately 100 total bands are needed to adequately portray these relationships. Cultivars of cabbage and cauliflower were separated by RAPDs. Analysis of RAPDs from individual plants of B. carinata cv. dodola resulted in 69 RAPDs, with 91.7% monomorphic and 8.3% polymorphic bands. RAPDs appear to be useful for taxonomic studies at levels ranging from populations to species and perhaps genera.
Bibliography:F30
92B0624
ISSN:0040-5752
1432-2242
DOI:10.1007/bf00227415