Detection of Treponema phagedenis–like antibodies in serum and bulk milk from cows with and without digital dermatitis

• Published in: Journal of veterinary diagnostic investigation Vol. 30; no. 1; pp. 86 - 92
• Main Authors: Frössling, Jenny, Rosander, Anna, Björkman, Camilla, Näslund, Katarina, Pringle, Märit
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.01.2018
• Subjects: Animals; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - microbiology; Clinical Science; Digital Dermatitis - diagnosis; Digital Dermatitis - microbiology; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Female; Full Scientific Reports; Klinisk vetenskap; Medical Bioscience; Medicinsk biovetenskap; Milk - microbiology; Sensitivity and Specificity; Treponema - isolation & purification; Treponemal Infections - diagnosis; Treponemal Infections - microbiology; Treponemal Infections - veterinary; cattle; Bovine digital dermatitis; enzyme-linked immunosorbent assay; Treponema
• ISSN: 1040-6387; 1943-4936; 1943-4936
• DOI: 10.1177/1040638717733778

Digital dermatitis (DD) in dairy cows is a widespread disease linked to infection with Treponema. The traditional diagnostic method is clinical inspection, which is subjective and laborious. We explored the performance of 4 different immunogenic proteins from Treponema phagedenis in a new antibody ELISA for analysis of serum or milk. Analysis of samples from 390 cows in 25 herds showed that the ELISA could distinguish the majority of cows with DD from healthy cows. By changing the cutoff and applying parallel or serial testing, high sensitivity or specificity could be achieved. The investigation indicated that aggregated test results can be useful in the assessment of a herd’s DD status. In addition, analysis of bulk tank milk samples showed good agreement with results from individual cows. The test system could be useful in research on the epidemiology and immunology of DD.