Nitrate reduction in pea plants: Effects of nitrate application and RHIZOBIUM strains

• Published in: Soil biology & biochemistry Vol. 23; no. 7; pp. 695 - 699
• Main Authors: Hervas, A., Ligero, F., Lluch, C.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1991; New York, NY Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Economic plant physiology; Fundamental and applied biological sciences. Psychology; nitrate; nitrate reductase (NADH); nutrient availability; Rhizobium leguminosarum; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Symbionte; Enzyme; Vegetal microorganism relation; Nodulation; Nitrates; Rhizobium leguminosarum; Proteins; Grain legume; Leguminosae; Enzymatic activity; Nitrate reductase; Dicotyledones; Pisum sativum; Angiospermae; Nitrogen fixation; Bacteria; Spermatophyta; Rhizobiaceae; Biological accumulation
• ISSN: 0038-0717; 1879-3428
• DOI: 10.1016/0038-0717(91)90085-X

Growth, nitrate reductase activity (NRA; EC 1.6.6.1), nitrate content and soluble protein concentration were studied in pea ( Pisum sativum cv. Lincoln) plants supplied with different NOf concentrations and inoculated with selected Rhizobiwn leguminosarum strains. Non-inoculated plants grown in the same nutrient medium served as controls. NRA was determined by an in vivo (−NO 3 −) assay. Differences in tissue NRA were mainly related to NO 3 − concentration in the growth medium; however, nodulation markedly influenced the amounts of NRA in different plant organs, especially roots. The concentration of NO 3 − in leaves, stems and roots increased markedly with NO 3 − dose. Although the differences in the NO 3 − accumulation were mainly ascribed to NO 3 − treatment, nodulation also affected the NO 3 − content of tissues, especially of roots and stems.