Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

• Published in: Bioenergy research Vol. 15; no. 1; pp. 399 - 411
• Main Authors: Kutyła, Mateusz, Trytek, Mariusz, Buczek, Katarzyna, Tomaszewska, Ewa, Muszyński, Siemowit
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2022; Springer; Springer Nature B.V
• Subjects: Acetates; Acetic acid; Batch culture; Biocatalysts; Biomass; Biomedical and Life Sciences; Biosynthesis; Calcium carbonate; Catalytic activity; Citronellol; Citronellyl acetate; Energy consumption; Esterification; Fungi; Hexanes; Life Sciences; Lipase; Low temperature; Mycelia; n-Hexane; Olive oil; Organic acids; Physiological aspects; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant Sciences; Substrates; Wood Science & Technology; Citronellyl acetate; Biomass; Biomass-bound lipase; Esterification; Psychrophiles
• ISSN: 1939-1234; 1939-1242
• DOI: 10.1007/s12155-021-10289-x

A biomass-bound lipase from psychrophilic Chrysosporium pannorum A-1 is an efficient biocatalyst for direct esterification of β-citronellol and acetic acid in an organic solvent. The biomass is effectively produced by fungal submerged culture at 20 ℃, which results in lower energy consumption during the production of biocatalyst. Supplementation of the culture medium with calcium carbonate together with olive oil contributed to a significant increase in the active biomass of mycelium in one batch culture and increased the efficiency of the biocatalyst. Biomass-bound lipase showed high catalytic activity in a broad temperature range of 30–60 °C and stability up to 70 °C. A maximum molar conversion value of 98% was obtained at 30 °C in n -hexane using a 2:1 alcohol-to-acid molar ratio and 3% w/v of the biocatalyst within 24 h. The high equimolar concentration of the substrates (200 mM) did not have an adverse effect on mycelial biomass activity. Dry mycelium of C. pannorum is a promising biocatalyst for large-scale biosynthesis of citronellyl acetate, given its low-cost production, high activity at low temperatures, and reusability in a minimum of seven 24-h biocatalytic cycles.