Biotransformation of d-sorbitol to l-sorbose by immobilized cells Gluconobacter suboxydans in a bubble column

• Published in: Journal of biotechnology Vol. 19; no. 2; pp. 301 - 307
• Main Authors: Kosseva, M., Beschkov, V., Popov, R.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.07.1991; Amsterdam Elsevier; New York, NY
• Subjects: Bioconversions. Hemisynthesis; Biological and medical sciences; bioreactors; Biotechnology; Bubble column; D-sorbitol; d-Sorbitol biotransformation; Fundamental and applied biological sciences. Psychology; Gluconobacter suboxydans; Immobilized cell; immobilized cells; L-sorbose; Methods. Procedures. Technologies; oxygen; Volumetric mass transfer coefficient; Immobilized cell; Volumetric mass transfer coefficient; Gluconobacter suboxydans; Bubble column; d-Sorbitol biotransformation; Pseudomonadales; Sorbitol; Bioreactor; Gluconobacter oxydans suboxydans; Biotransformation; Sorbose; Bacteria; Pseudomonadaceae
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/0168-1656(91)90066-5

The present study investigates the d-sorbitol to l-sorbose biotransformation carried out in a bubble column bioreactor by the bacteria Gluconobacter suboxydans, entrapped in a gel of calcium alginate. The volumetric coefficient for oxygen mass transfer is evaluated too. It appeared that the higher the dry substances concentration is the more rapidly the process runs. This effect is explained by the autocatalytic pattern of the biochemical process and the effect of substrate inhibition. The K la-values are close to the ones observed for a free cell culture. Thus a bubble column bioreactor could be successfully used for the studied process instead of a stirred tank reactor.