CGRP stimulates gill carbonic anhydrase activity in molluscs via a common CT/CGRP receptor

• Published in: Peptides (New York, N.Y. : 1980) Vol. 27; no. 11; pp. 2678 - 2682
• Main Authors: Cudennec, Benoit, Rousseau, Marthe, Lopez, Evelyne, Fouchereau-Peron, Martine
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.11.2006; Elsevier Science; Elsevier
• Subjects: AC187; Animals; Biological and medical sciences; Calcification, Physiologic - drug effects; Calcification, Physiologic - physiology; Calcitonin - pharmacology; Calcitonin Gene-Related Peptide - pharmacology; Calcitonin Gene-Related Peptide - physiology; Carbonic anhydrase; Carbonic Anhydrases - drug effects; Carbonic Anhydrases - metabolism; Cell Behavior; Cellular Biology; CGRP; CGRP8-37; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Gill; Gills - drug effects; Gills - enzymology; Humans; Life Sciences; Peptide Fragments - pharmacology; Peptide Fragments - physiology; Pinctada - drug effects; Pinctada - enzymology; Pinctada - metabolism; Receptors, Calcitonin Gene-Related Peptide - metabolism; Salmon; Time Factors; Vertebrates: endocrinology; CT; Gill; CGRP8-37; Carbonic anhydrase; CGRP; AC187; Enzyme; Lyases; Calcitonin gene related peptide; Neuropeptide; Carbonate dehydratase; CGRP receptor; Invertebrata; Mollusca; Carbon-oxygen lyases; Hydro-lyases; carbonic anhydrase; gill
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2006.05.019

The physiological significance of calcitonin gene-related peptide (CGRP) during biomineralization was investigated by assessing the effect of human CGRP on the carbonic anhydrase activity in gill membranes of the pearl oyster, Pinctada margaritifera. Salmon CT and human CGRP were able to induce a 150% increase of the basal activity. No additive effect was observed suggesting that both activities are mediated by the same receptor. The CGRP-stimulated effect was specific as demonstrated by the inhibition produced by the CGRP antagonist, hCGRP8-37. So, CGRP by its specific action on gill carbonic anhydrase controls the calcification process, an ancient role both in invertebrates and non-mammalian vertebrates.