Slackia equolifaciens sp. nov., a human intestinal bacterium capable of producing equol

• Published in: International journal of systematic and evolutionary microbiology Vol. 60; no. Pt 8; pp. 1721 - 1724
• Main Authors: JIN, Jong-Sik, KITAHARA, Maki, SAKAMOTO, Mitsuo, HATTORI, Masao, BENNO, Yoshimi
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.08.2010
• Subjects: Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Actinobacteria - metabolism; Bacteriology; Biological and medical sciences; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Equol; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Intestines - microbiology; Isoflavones - metabolism; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Systematics; Human; Bacteria; New species; Digestive system; Taxonomy; Gut
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.016774-0

An equol-producing bacterium, strain DZE(T), which was isolated from human faeces, was characterized by morphological, biochemical and molecular methods. The isolate was Gram-positive, obligately anaerobic, non-spore-forming, asaccharolytic and rod-shaped. 16S rRNA gene sequence analysis showed 92.8, 91.0, 91.1 and 90.6% similarities with Slackia faecicanis, Slackia exigua, Slackia heliotrinireducens and Slackia isoflavoniconvertens, respectively. Based on these data, we propose a novel species of the genus Slackia, Slackia equolifaciens sp. nov. The major cellular fatty acids are C14:0, C18:1omega9c and C18:1omega9c DMA (dimethyl acetal). The DNA G+C content of the strain is 60.8 mol%. The type strain of S. equolifaciens sp. nov. is DZET (=JCM 16059T =CCUG 58231T).