Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection

Tuberculosis (TB) is the most lethal infection among infectious diseases. The specific aim of this study was to establish panels of serum protein biomarkers representative of active TB patients and their household contacts who were either infected (LTBI) or uninfected (EMI-TB Discovery Cohort, Ponte...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 10; no. 1; p. 3844
Main Authors Mateos, Jesús, Estévez, Olivia, González-Fernández, África, Anibarro, Luis, Pallarés, Ángeles, Reljic, Rajko, Mussá, Tufária, Gomes-Maueia, Cremildo, Nguilichane, Artur, Gallardo, José M, Medina, Isabel, Carrera, Mónica
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 02.03.2020
Nature Publishing Group UK
Subjects
Adult
Apolipoprotein A
Complement activation
Contact Tracing
Female
Humans
Hydrogen-Ion Concentration
Immune response
Immunomodulation
Infectious diseases
Male
Middle Aged
Nephelometry
Proteins
Proteomes
Proteomics
Tuberculosis
Tuberculosis - blood
Tuberculosis - metabolism
Tuberculosis - transmission
Online AccessGet full text

Cover

More Information
Summary:Tuberculosis (TB) is the most lethal infection among infectious diseases. The specific aim of this study was to establish panels of serum protein biomarkers representative of active TB patients and their household contacts who were either infected (LTBI) or uninfected (EMI-TB Discovery Cohort, Pontevedra Region, Spain). A TMT (Tamdem mass tags) 10plex-based quantitative proteomics study was performed in quintuplicate containing a total of 15 individual serum samples per group. Peptides were analyzed in an LC-Orbitrap Elite platform, and raw data were processed using Proteome Discoverer 2.1. A total of 418 proteins were quantified. The specific protein signature of active TB patients was characterized by an accumulation of proteins related to complement activation, inflammation and modulation of immune response and also by a decrease of a small subset of proteins, including apolipoprotein A and serotransferrin, indicating the importance of lipid transport and iron assimilation in the progression of the disease. This signature was verified by the targeted measurement of selected candidates in a second cohort (EMI-TB Verification Cohort, Maputo Region, Mozambique) by ELISA and nephelometry techniques. These findings will aid our understanding of the complex metabolic processes associated with TB progression from LTBI to active disease.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-60753-5